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Gene expression profile at single cell level of OT-I CD8+ T cells from B16-OVA melanoma tumors

ABSTRACT: Tumor infiltrating T cells are a diverse and heterogeneous population. We used single cell RNA sequencing (scRNA-seq) to analyze gene expression changes in tumor-specific T cells that were sufficient for or lacked PSGL-1 (gene: Selplg).

ORGANISM(S): Mus musculus

PROVIDER: GSE226523 | GEO | 2023/04/03

REPOSITORIES: GEO

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Epigenetic profiling of OT-I CD8+ T cells after T cell activation

Project description:T cells undergo epigenetic changes upon T cell activation. We used the Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq) to analyze epigenetic changes in T cells that were sufficient for or lacked PSGL-1 (gene: Selplg) two hours after T cell activation.

2023-04-03 | GSE226521 | GEO

scRNA-seq comparison of B16-GP33 tumor-infiltrating immune cells from IgG, anti-PD-1, anti-PSGL-1, or combination anti-PD-1 and anti-PSGL-1 treated mice

Project description:We use scRNA-seq to show the differences in tumor-infiltrating immune cells among IgG, anti-PD-1, anti-PSGL-1, and combination anti-PD-1 and anti-PSGL-1 treated mice. We show that anti-PSGL-1 treatment resulted in an increase in neutrophil and T cells, anti-PD-1 treatment resulted in an increase in macrophages, and the combination resulted in an increase in T cells and macrophages when compared to the tumors of IgG treated mice. Additionally, we show that Tregulatory cells are decreased in the tumors of anti-PSGL-1 and combination treated mice. Further, we find that anti-PSGL-1 treated CD8 T cells show upregulation of activation and survival genes, while combination treatment increased effector gene expression in CD8 T cells. Both anti-PSGL-1 treatment and combination treatment increase effector gene expression in CD4 T cells when compared to IgG. This scRNA-seq study shows the impact of IgG, anti-PD-1, anti-PSGL-1, and combination anti-PSGL-1 and anti-PD-1 antibody tteatment on tumor-infiltrating immune cells in B16-GP33 melnoma tumor bearing mice.

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Gene Expression patterns in mouse B16-OVA melanoma tumors

Project description:This laboratory focuses on selectin mediated recruitment during adoptive immunotherapy for metastatic cancer. This study seeks to determine changes in the expression levels of Fucosyltransferases, Selectins, and cytokines in normal and inflamed mouse skin, melanoma tumor tissue of different sizes, and tumor cells grown in culture. Since the ability to treat the tumor effectively is directly related to the size of the tumor, differences in glyco-expression patterns may be of interest. In this study, five groups were hybridized and analyzed using the GLYCOv2 array. Each group was analyzed in triplicate. The groups were: Normal mouse skin, normal mouse skin inflamed by treatment with Oxazolone, B16-OVA melanoma tissue from 6 day tumors, B16-OVA melanoma tissue from 11 day tumors, and B16-OVA grown in cell culture.

2011-02-17 | E-GEOD-27355 | biostudies-arrayexpress

Gene Expression patterns in mouse B16-OVA melanoma tumors

2011-02-17 | GSE27355 | GEO

Transcriptomic analysis of CD11b+ DsRed+ CD45hi cells harvested from the ischemic brain of Selplg-KODsRed → WT chimeras and Selplg-WTDsRed→ WT chimeras at 7 days after 30 min MCAo/reperfusion

Project description:In order to assess whether PSGL-1 (SELPLG) deficiency impacts key characteristics of invading cells in our stroke model, we performed a detailed transcriptomic analysis of retrovirally transduced CD11b+ CD45hi cells harvested from the ischemic brain at 7 days after MCAo

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CXCR4 inhibition modulates the tumor microenvironment and retards the growth of B16-OVA melanoma and Renca tumors.

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Single-cell RNA-seq of sorted CD8+ T cells from B16 melanoma tumors

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Single-cell RNA-seq of sorted CD8+ T cells from B16 melanoma tumors

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10X single-cell RNASeq profiling of tumor-infiltrating CD8+ T-cells from B16-OVA mouse melanoma tumors

Project description:10X single-cell RNASeq profiling of tumor-infiltrating CD8+ T-cells from B16-OVA mouse melanoma tumors

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Next Generation Sequencing of CNS-infiltrating Tox-sufficient and -deficient OT-1 cells in ODC-OVA mice 7 days after LCMV-OVA infection

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2018-05-17 | GSE93804 | GEO

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