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Effect of HPV E5 on tumor on immune response and tumor development

ABSTRACT: To investigate the functions of HPV16 E5, we established empty vector or HPV16 E5-transduced CAL-27 and DC2.4 cell lines. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells lines.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE226754 | GEO | 2024/01/29

REPOSITORIES: GEO

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E5 human papillomavirus type 16 oncoprotein modify ST3GAL3 and ST6GAL1 gene expression

Project description:Human papillomavirus (HPV) is the etiological agent of cervical cancer. Three viral proteins, E5, E6 and E7 have been implicated in cell transformation. Increased expression of sialic acid and sialylated antigens have been reported during cervix transformation, these results agree with the increased mRNA levels of the sialyltransferases genes ST6GAL1 and ST3GAL3 reported in premalignant and malignant tissue of the cervix. E6 and E7 HPV oncoproteins modify the expression of some glycogenes. The role of E5 HPV oncoprotein in the glycogene expression changes in premalignant and malignant cervical tissue has not been reported. The objective of this work was to identify glycogenes that modify their expression by E5 HPV oncoprotein in HaCaT cell line. A gene expression microarray was performed on HaCaT cells that stably expressed the HPV16 E5 oncogene. Analysis revealed alteration in some glycogenes including upregulation of ST6GAL1 and ST3GAL3. The increased mRNA levels of both genes were confirmed by qRT-PCR. In addition, an in-silico analysis was performed to identify glycosylation networks altered in presence of E5 oncoprotein. The analysis showed that E5 could modify the sialic acid expression, keratan sulfate synthesis, N-glycosylation and biosynthesis of glycosaminoglycans. This is the first report of the role of HPV16 E5 oncoprotein on glycogenes expression changes. Moreover, our results suggest that the increase of the sialyltransferases genes reported in premalignant and malignant cervical tissue, could be the result of the expression of E5 oncoprotein. These results provide information of the possible role of HPV infection on the sialylation changes in the cervical epithelium identified in premalignant lesions and cancer.

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C/EBPα is crucial determinant of epithelial maintenance by preventing epithelial-to-mesenchymal transition

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Expression data from AKR1C1 over-expression in Cal-27 cells or knockdown in HSC-2 cells, respectively

Project description:To exmaine the AKR1C1 controls transcriptome in HNSCC cells, we preformed the Affymetrix Human Genome U133 Plus 2.0 Array with empty vector or AKR1C1 expression vector in Cal-27 cells and shLuc control or shAKR1C1 in HSC-2 cells

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Self-Enforcing Feedback Activation between BCL6 and Pre-B Cell Receptor Signaling Defines a Distinct Subtype of Acute Lymphoblastic Leukemia

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2018-02-12 | GSE83742 | GEO

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