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RNA sequencing of enterocytes differentiated from human iPS cells

ABSTRACT: Our RNA-seq results showed that valproic acid (VPA) works synergistically with activated Vitamin D3 (VD3) to enhance the expression of enterocyte-function-related genes in iPS-derived enterocyte-like cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226851 | GEO | 2023/05/25

REPOSITORIES: GEO

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RNA sequencing of enterocytes differentiated from human iPS cells [RNAseq1]

Project description:Our RNA-seq results show the changes in gene expression during differentiation and maturation of iPS-derived enterocytes, as well as that treatment during the last stage with Vitamin D3 (VD3) enhanced enterocyte-related gene expression. Caco-2 and RNA from human adult small intestine were also sequenced as comparison.

2023-05-25 | GSE226661 | GEO

RNA sequencing of enterocytes differentiated from human iPS cells

Project description:RNA sequencing of enterocytes differentiated from human iPS cells

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Project description:Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, the efficiency of biallelic genetic engineering in transcriptionally inactive genes is still low, unlike that in transcriptionally active genes. To enhance the biallelic homologous recombination efficiency in human ES/iPS cells, we performed screenings of accessorial genes and compounds. We found that RAD51 overexpression and valproic acid treatment enhanced biallelic-targeting efficiency in human ES/iPS cells regardless of the transcriptional activity of the targeted locus. Importantly, RAD51 overexpression and valproic acid treatment synergistically increased the biallelic homologous recombination efficiency. Our findings would facilitate genome editing study using human ES/iPS cells.

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RNA sequencing of enterocytes differentiated from human iPS cells [RNAseq1]

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Genome-wide gene expression prolifing post-valproic acid exposure in human forebrain organoids

Project description:Human forebrain organoids (day 42) derived from U1M and U2F iPS cell lines were exposed with or without 1 mM valproic acid (VPA) for 72h. Organoids were then randomly selected for RNA sequencing, we found that differentially expressed genes (DEGs) induced by VPA exposure that shared in both U1M and U2F were enriched with neural development, synaptic transmission, calcium and potassium signaling pathways. The DEGs were also significantly enriched with autism spectrum disorder (ASD)-associated genes, including genes dysregulated in brains or organoids derived from ASD patients, and known ASD risk genes, as well as genes in ASD risk-associated gene coexpression modules.

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human placenta-derived cell line (JEG-3): Control vs. valproic acid(VPA)-treated

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Project description:tMCAO+ Valproic acid (VPA)

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2023-05-10 | PXD040622 | Pride

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