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Expression analysis of laser cut germaria vs laser cut apex of testes

ABSTRACT: Here we used laser cutting microdissection and RNA amplification to profile the gene expression in wildtype female germaria and male apex of the testes. These tissue contain germline stem cells and early dividing germ cells. Our goal was to identify genes expressed in these cell types.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE22686 | GEO | 2012/01/18

SECONDARY ACCESSION(S): PRJNA128341

REPOSITORIES: GEO

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Expression analysis of laser cut germaria vs laser cut apex of testes

Project description:Here we used laser cutting microdissection and RNA amplification to profile the gene expression in wildtype female germaria and male apex of the testes. These tissue contain germline stem cells and early dividing germ cells. Our goal was to identify genes expressed in these cell types. A direct microarray design of laser cut germaria vs laser cut apex of testes. Four biological replicates are included with two dye-swaps.

2012-01-18 | E-GEOD-22686 | biostudies-arrayexpress

Gene expression profiles in 74 laser microdissected colorectal cancer tissues

Project description:Tissues from another series of 74 patients with colorectal cancer were collected by laser micro-dissection with the Leica Laser Microdissection System (Leica Microsystems).

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Gene expression profiles in 132 laser microdissected colorectal cancer tissues

Project description:Tissues from another series of 132 patients with colorectal cancer were collected by laser micro-dissection with the Leica Laser Microdissection System (Leica Microsystems).

2011-09-01 | GSE21815 | GEO

Drosophila melanogaster

Project description:Expression analysis of laser cut germaria vs laser cut apex of testes

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Expression data from laser microdissected murine intestinal epithelial cells

Project description:Microarray analyses of laser microdissected murine intestinal epithelial cells under the control of maternal inflammation at 17.5 days post conception demonstrates that maternal inflammation differentially regulates 2174 (iWT) and 3345 (iARE) genes in fetal tissue, but these transcriptional profiles were overwritten in the postnatal environment dominated by tissue inflammation at 8 weeks postnatal. We isolated 50 ng total RNA out of laser microdissected fetal and adult intestinal epithelial cells from the distal ileum and performed microarrays to address whether maternal inflammation programs the fetal intestine towards TNF-driven pathology Conventionally raised heterozygous TnfM-NM-^TARE/+ and Tnf+/+ wild type mice on C57BL/6 background were used for this study. Female Tnf+/+ wild type (WT) mice were bred with male TnfM-NM-^TARE/+ (ARE) mice and vice versa, generating offspring from healthy WT dams (WT and ARE) and inflamed ARE dams (iWT and iARE). On day 17.5 post conception (dpc) and 8 weeks postnatal the fetuses and offspring were sacrificed.The gut was freshly frozen in Optimal Cutting Temperature (O.C.T.) embedding compound (Sakura Finetek, Torrance, USA) on dry ice and stored at -80M-BM-0C until laser microdissection.

2014-05-05 | E-GEOD-44433 | biostudies-arrayexpress

Profiling of APEX-NLS and APEX-PMLca associated regions

Project description:To address specificity of ALaP-seq for PMLwt, we performed genome-wide profiling of genomic regions associated with NLS-APEX or PMLca-APEX. Cells were treated with H2O2 to trigger labeling of chromatin with biotin (H2O2+). Experimental negative control for PMLca (H2O2-), where the H2O2 treatment was omitted, was also analyzed.

2020-01-10 | GSE143362 | GEO

Profiling of APEX-NLS and APEX-PMLca associated regions

Project description:Profiling of APEX-NLS and APEX-PMLca associated regions

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Laser Capture Microdissection (LCM) analysis of mycorrhizal rice roots

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Laser Capture Microdissection and RNA Sequencing Human Adamantinomatous Craniopharyngioma

Project description:Laser Capture Microdissection and RNA Sequencing Human Adamantinomatous Craniopharyngioma

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Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex

Project description:We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology. Keywords: cell type comparison, laser capture microdissection

2008-12-18 | GSE12679 | GEO

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