ScRNAseq analysis of four populations sorted from midbrain organoids using Flow cytometry

ABSTRACT: Midbrain organoid derived from human induced pluriopotent stem cells a new model of Parkinson's Disease. Organ specific organoids can be used to model many diseases, however little is know about these models. We created a flow cytometry workflow to identify cell types in midbrain organoids. We then selected four populations and sorted these populations: Neurons1(CD24++), Neurons2(CD56++), Astrocytes and Radial Glia, using FACS and the antibody intensity gates defined by our workflow. We then performed scRNAseq on the sorted population and identified cell types within the sorted populations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226890 | GEO | 2023/03/15

REPOSITORIES: GEO