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The intracellular domain of amyloid precursor protein induces the dynamic change of the gene expression profile in P19 cells.

ABSTRACT: As previously reported (Neuroscience letters 444(2008)127-131), we established embryonic carcinoma P19 cell line expressing the intracellular domain of APP (AICD). Although neurons could be differentiated from these cell lines with RA treatment, expression of AICD gave rise to neuron-specific apoptosis. To identify the genes that are involved in this cell death, we evaluated changes of gene expressions that was induced by AICD through this process of cell death. The expression profiles of almost forty thousands transcripts were monitored by DNA microarrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE22690 | GEO | 2010/07/14

SECONDARY ACCESSION(S): PRJNA128295

REPOSITORIES: GEO

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The intracellular domain of amyloid precursor protein induces the dynamic change of the gene expression profile in P19 cells.

Project description:As previously reported (Neuroscience letters 444(2008)127-131), we established embryonic carcinoma P19 cell line expressing the intracellular domain of APP (AICD). Although neurons could be differentiated from these cell lines with RA treatment, expression of AICD gave rise to neuron-specific apoptosis. To identify the genes that are involved in this cell death, we evaluated changes of gene expressions that was induced by AICD through this process of cell death. The expression profiles of almost forty thousands transcripts were monitored by DNA microarrays. AICD-expressing P19 cell lines (AICD/P19) and control P19 cell lines (pCDNA/P19) that carry vector alone were used and cultured. To induce neural differentiation of these P19 cell lines, cells were allowed to aggregate in culture medium with 5×10-7 M all-trans-retinoic acid (RA) for 4 days (aggregated state). After aggregation, cells were replated onto cell culture grade dishes or poly-L-Lysine coated cell-disks for two days (differentiated state). RNAs were isolated at each states.

2010-07-14 | E-GEOD-22690 | biostudies-arrayexpress

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Project description:Here we mapped peptides of mouse SRSF7_RRM purified from P19 cells.

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P19 embryonic carcinoma (EC) cells induced to form cardiomyocytes in vitro

Project description:SAGE identification of differentiation responsive genes in P19 embryonic cells induced to form cardiomyocytes in vitro. P19 embryonic carcinoma (EC) cells, induced to form cardiomyocytes in vitro - undifferentiated cells, day 3+0.5 and day 3+3.0 of differentiation protocol. Keywords = EC cells, P19, differentiation, cardiomyocytes Keywords: time-course

2002-07-18 | GSE42 | GEO

Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion.

Project description:To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells.

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Peptide mapping of SRSF7_RS-GFP isoforms in murine P19 cells

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P19-atRA-kinetics

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MicroRNA profiling of human HeLa cells overexpressing p19 H-Ras

Project description:MicroRNA miRNA expression profiles for human HeLa cells (Cervical cancer), overexpressing p19 H-Ras, were examined to investigate the miRNA regulation by p19 H-Ras. miRNA microarray analysis identified statistical unique profiles, which could discriminate miRNAs regulated by p19 H-Ras and not regulated by the p19 mutant (W164A) H-Ras.

2008-12-12 | E-TABM-494 | biostudies-arrayexpress

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