Transcriptomics

Dataset Information

39

Systems analysis of the Merck Ad5/HIV vaccine reveals robust induction of a core innate immune gene network: in vitro analysis


ABSTRACT: Here we applied a systems approach to define human innate immune signatures following MRKAd5/HIV immunization and to analyze the effects of pre-existing Ad5 immunity. To determine whether vaccine-induced changes could be ascribed to cell-intrinsic responses rather than cell population fluxes, fresh PBMC from independent Ad5Neg donors were stimulated in vitro with MRKAd5 vector lacking HIV-1 transgenes (MRKAd5). Overall design: 22 total samples were analyzed. 14 of these are from in vivo vaccination of seven Ad5 seronegative subjects at two time points (0hr and 24hrs). 8 of these are from 24hr in vitro stimulation of PBMCs from four independent Ad5 seronegative donors with MRKAd5 empty vector or GTS buffer (Mock) at 20,000 viral particles per cell.

INSTRUMENT(S): Affymetrix Human Exon 1.0 ST Array [CDF: Brainarray V 9.0, HsEx10stv2_Hs_ENTREZG]

ORGANISM(S): Homo sapiens  

SUBMITTER: Daniel Edward Zak 

PROVIDER: GSE22769 | GEO |

SECONDARY ACCESSION(S): PRJNA129111

REPOSITORIES: GEO

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