Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic profile of dermal and hypodermal CCR2+ macrophages, CCR2- macrophages and dendritic cells from C57BL/6 mice

ABSTRACT: In order to explore and compare the transcriptomic profile of CCR2+ macrophages, CCR2- macrophages and dendritic cells present in the dermis and hypodermis of C57BL/6 mice, isolated cells were subjected to RNA-sequencing analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE227758 | GEO | 2023/09/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

IL10RA signaling deficiency change the anti-inflammatory signature of Ccr2-independent resident macrophages

Project description:In adult mice the majority of intestinal macrophages exhibit a mature phenotype and are derived from blood monocytes. In the steady state replenishment of these cells is reduced in the absence of the chemokine receptor Ccr2. Within the intestine of mice with colitis there is marked increase in the accumulation of immature macrophages that demonstrate an inflammatory phenotype. However, whether Ccr2 is necessary for the accumulation of these immature macrophages and further for the development of colitis in susceptible mouse models is incompletely defined. Here, we ask whether Ccr2 is necessary for the development of colitis in mice lacking the receptor for IL10. We compared the development of intestinal inflammation in mice lacking IL10RA or both IL10RA and Ccr2. The absence of Ccr2 interfered with the accumulation of immature macrophages in IL10R-deficien mice, including a novel population of rounded submucosal Iba1+ cells and reduced the severity of colitis in these mice. In contrast, the absence of Ccr2 did not reduce augmented inflammatory gene expression observed in mature intestinal macrophages isolated from mice lacking IL10RA. These data suggest that both newly recruited Ccr2-dependent immature macrophages and Ccr2-independent residual mature macrophages contribute to the development of intestinal inflammation observed in IL10R-deficient mice.

2022-01-19 | GSE183960 | GEO

Dermal and hypodermal macrophages and dendritic cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-06 | GSE228445 | GEO

CCR2+ vs. CCR2- murine hematopoietic stem cells

Project description:Comparative analysis of FACS-sorted CCR2- and CCR2+ HSC in the steady state. CCR2+ HSC have fourfold higher proliferative rates than CCR2- HSC, are are biased towards the myeloid lineage and dominate the migratory HSC population. Comparison of pooled CCR2- and CCR2+ HSC (bone marrow from 20 mice pooled for each sample), three biological replicates each.

2015-05-01 | E-GEOD-53827 | biostudies-arrayexpress

Expression data of murine dermal macrophages

Project description:Dermal macrophages were sorted from the ears of CCR2-/- mice that were uninfected, injected with PBS or injected with S.aureus strain Newmann 107 CFU/ml live S. aureus was injecetd in 10µl PBS.

2020-07-15 | GSE145094 | GEO

Expression data from CCR2+ and CCR2- splenic marophages from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

Project description:We used microarrays to detail the global gene expression in CCR2+ and CCR2- spenic macrophages (SM) sorted from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

2019-01-01 | GSE97790 | GEO

Ccr2+ monocyte-derived macrophages influence trajectories of acquired therapy resistance in Braf-mutant melanoma

Project description:Therapies targeting oncogene addiction have had a tremendous impact on tumor growth and patient outcome, but drug resistance continues to be problematic. One approach to deal with the challenge of resistance entails extending anti-cancer treatments beyond targeting cancer cells by additionally altering the tumor microenvironment. Understanding how the tumor microenvironment contributes to the evolution of diverse resistance pathways could aid in the design of sequential treatments that can elicit and take advantage of a predictable resistance trajectory. Tumor associated macrophages are often the most abundant immune cell found in tumors. Here, we used clinically relevant in vivo Braf-mutant melanoma models with fluorescent markers to track the stage-specific changes in macrophages under targeted therapy with Braf/Mek inhibitors and assessed the dynamic evolution of the macrophage population generated by therapy pressure-induced stress. During the onset of a drug-tolerant persister state, Ccr2+ monocyte-derived macrophage infiltration rose, suggesting that macrophage influx at this point could facilitate the onset of stable drug resistance after several weeks of treatment. Comparison of melanomas that develop in a Ccr2-proficient or deficient microenvironment demonstrated that lack of melanoma infiltrating Ccr2+ macrophages delayed onset of resistance and shifted melanoma cell evolution towards unstable resistance. Unstable resistance was characterized by sensitivity to targeted therapy when factors from the microenvironment were lost. Importantly, this phenotype was reversed by co-culturing melanoma cells with Ccr2+ macrophages. Overall, this study demonstrates that the development of resistance may be directed by altering the tumor microenvironment to improve treatment timing and the probability of relapse.

2023-05-09 | GSE226835 | GEO

Single-cell transcriptomic profile of CD45+ and CD45- cells from dermis and hypodermis of C57BL/6 mice

Project description:In order to explore and compare the transcriptomic profile of leucocytes and stromal cells present in the dermis and hypodermis of C57BL/6 mice, isolated cells from each of these skin layers were subjected to single-cell RNA-sequencing analysis.

2023-09-06 | GSE228018 | GEO

CCR2+ vs. CCR2- murine hematopoietic stem cells

2015-05-01 | GSE53827 | GEO

Activin A and CCR2 regulate the function of macrophages in testicular fibrosis caused by experimental autoimmune orchitis

Project description:Treatment of bone marrow-derived macrophages with activin A in vitro increased the expression of Ccr2, Fn1, Cxcr4, and Mmp2 and these effects were abolished by follistatin.

2024-04-19 | GSE210004 | GEO

Monocytes transition to monocyte-macrophages within the inflamed vasculature via CCR2 on monocytes and endothelial TNFR2

Project description:Our study reports that CCR2 dependent functional specialization of monocytes into immature macrophages occurs within the TNF-TNFR2 activated vasculature and establishes a chemokine-based autocrine, feed-forward loop that may aplify the inflammation and renal injury.

2022-02-21 | GSE196730 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data