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Effect of BAF disruption on chromatin accessibility in activated CD8+ T cells [ATAC-seq]

ABSTRACT: To investigate the role of cBAF in regulation of Tbet binding, polyclonal naive CD8 T cells were stimulated for 48h with anti-CD3, CD28, and hIL-2. Cells were then treated with DMSO, ACBI1, or BRM014 for 6 hours, and treated with recombinant mouse IL-12 for the last two hours of inhibitor treatment. Chromatin accessibility profiling was performed via ATAC-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE228193 | GEO | 2023/06/13

REPOSITORIES: GEO

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Effect of BAF disruption on T-bet binding in activated CD8+ T cells [ChIP-seq]

Project description:To investigate the role of cBAF in regulation of Tbet binding, polyclonal naive CD8 T cells were stimulated for 48h with anti-CD3, CD28, and hIL-2. Cells were then treated with DMSO, ACBI1, or BRM014 for 6 hours, and treated with recombinant mouse IL-12 for the last two hours of inhibitor treatment. ChIP-seq against T-bet was then performed.

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A non-canonical SWI/SNF complex underlies synthetic lethality in cancers driven by BAF complex perturbation

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BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (Microarray)

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BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (ATAC I)

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BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (RNA-seq II)

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Project description:To investigate the role of ARID1A in the regulation of transcription factor binding and histone modifications in effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a KO mice. We then performed CUT&RUN against ARID1A, BATF, ETS, Tbet, H3K27ac, H3K27me3 in WT, Arid1a KO, or Tbx21 (Tbet) KO cells in vivo at d0, 48h in vitro activated, d5, and d8 post-LCMV Armstrong infection.

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