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Characterization of a Medicago truncatula P450 monooxygenase mutant

ABSTRACT: The aim of the experiment was to characterise a P450 monooxygenase mutant involved in the biosynthetic pathway of haemolytic saponins. Saponins are a group of glycosidic compounds with an antimicrobial function. The gene CYP716A12 is responsible for an early step in saponin biosythesis. Transcriptome analysis of a CYP716A12 mutant was performed to investigate its function.

ORGANISM(S): Medicago sativa Sinorhizobium meliloti Medicago truncatula

PROVIDER: GSE22835 | GEO | 2010/12/29

SECONDARY ACCESSION(S): PRJNA127891

REPOSITORIES: GEO

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Characterization of a Medicago truncatula P450 monooxygenase mutant

Project description:The aim of the experiment was to characterise a P450 monooxygenase mutant involved in the biosynthetic pathway of haemolytic saponins. Saponins are a group of glycosidic compounds with an antimicrobial function. The gene CYP716A12 is responsible for an early step in saponin biosythesis. Transcriptome analysis of a CYP716A12 mutant was performed to investigate its function. 6 samples were used in the experiment: 3 biological replicates of CYP716A12 mutant plants and 3 biological replicates of wild-type plants.

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