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Single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity across six zebrafish organs

ABSTRACT: Tissue-resident macrophages (TRMs) and dendritic cells (DCs) are highly diverse and play essential roles in immunity, tissue regeneration, and homeostasis maintenance. To systematically assess the heterogeneity of TRM and DC in adult zebrafish, we performed droplet-based scRNA-seq on mpeg1.1+ cells sorted from six different organs (epidermis, gill, intestine, liver, heart, and brain) of 6-month-old Tg(mpeg1.1:DsRedx) fish, a widely used myeloid cell reporter line. We identified two macrophage subpopulations with pro-inflammatory and pro-remodeling signatures, respectively, as well as one conventional dendritic cell population with prominent antigen presentation capacity and plasmacytoid dendritic cells featuring anti-viral properties. Our study provides a comprehensive landscape of TRMs and DCs in zebrafish and offers valuable tools for future in-depth investigations of these cell types.

ORGANISM(S): Danio rerio

PROVIDER: GSE228806 | GEO | 2023/06/27

REPOSITORIES: GEO

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