Transcriptome-wide deamination analysis

ABSTRACT: Adenosine base editing to recreate the HPFH mutations is a promising approach to induce HbF. To measure Cas-independent off-target editing of mRNA, we electroporated CD34+ cells from three different donors with RNP consisting of ABE7.10 or ABE8e complexed with the -globin −175 A>G targeting sgRNA. Six days later, this was followed by high-depth RNA-seq. The rates of A-to-I conversion were similar in edited and unedited control cells, which is consistent with previous reports that off-target RNA editing was not detected after high-level adenine base editing of HUDEP2 cells or HSPCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE228821 | GEO | 2023/04/28

REPOSITORIES: GEO