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Effect of acute pyrvinium pamoate treatment on gene expression of CD4+ T cells during early activation.

ABSTRACT: Treatment of T cells with pyrvinium pamoate (PP) early during their activation blocks mitochondrial biogenesis and results in reduced proliferation, skewed CD4+ T cell differentiation and reduced cytokine production. Therefore, to examine the role of PP treatment on mitochondria health, we performed gene expression analysis to identify differentially expressed genes in naive CD4+ T cells cultured with anti-CD3/CD28 plus PP or DMSO. Naive CD4+ T cells were activated for six hours with 2 mg/mL anti-CD3/anti-CD28 mAbs and activated for an additional one hour in the presence or absence of PP.

ORGANISM(S): Mus musculus

PROVIDER: GSE229494 | GEO | 2023/08/16

REPOSITORIES: GEO

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