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Neurospora crassa early sexual development with oligod(T) priming

ABSTRACT: Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences.

ORGANISM(S): Neurospora crassa

PROVIDER: GSE22972 | GEO | 2010/07/17

SECONDARY ACCESSION(S): PRJNA133085

REPOSITORIES: GEO

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Saccharomyces cerevisiae grown in nitrogen depletion with oligodT priming

Project description:Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Saccharomyces cerevisiae S288c was grown on two different media and two different primers were used for reverse transcription: oligo(dT) and Multi-Targeted Primer [MTP] protoperithecia oligo(dT)+ MTP (PP2) perithecia oligo(dT)+ MTP (PT2). Two biological replicates and dye swapping

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Neurospora crassa early sexual development with MTP priming

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Multi-Targeted Priming For Genome-Wide Gene Expression Assays

Project description:This SuperSeries is composed of the following subset Series: GSE22658: Neurospora crassa early sexual development with MTP priming GSE22936: Saccharomyces cerevisiae grown in nitrogen depletion with MTP priming GSE22972: Neurospora crassa early sexual development with oligod(T) priming GSE22992: Saccharomyces cerevisiae grown in nitrogen depletion with oligodT priming Refer to individual Series

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