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Anti-cancer activity of verteporfin in cholangiocarcinoma

ABSTRACT: We investigated the antitumoral effect of verteporfin in YAP/AKT hydrodynamic tail vein injected murine models and we analyzed the correlative change of immune cell profile and stemness with the treatment of verteporfin by using flow cytometry and single-cell RNA sequencing

ORGANISM(S): Mus musculus

PROVIDER: GSE229855 | GEO | 2023/04/21

REPOSITORIES: GEO

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Single-cell transcriptomics reveal the dynamic of cancer stem-like cells during cholangiocarcinoma progression

Project description:We investigated YAP/AKT hydrodynamic tail vein injected murine models and we analyzed the correlative change of immune cell profile and stemness during cholangiocarcinoma progression using single-cell RNA sequencing

2023-12-01 | GSE233623 | GEO

Impact of Verteporfin on HCC cells

Project description:Together with TEAD4 the Hippo pathway effector YAP stimulates chromosomal instability. Verteporfin is known to disrupt the physical YAP/TEAD interaction and tehrefore might prevent from chromosomal instability in liver cancer. Immortalized HCC cell line hepG2 is treated with Verteporfin for 24 hours.

2016-02-11 | E-GEOD-73396 | biostudies-arrayexpress

Impact of Verteporfin on HCC cells

2016-02-11 | GSE73396 | GEO

Tumor selective proteotoxicity of verteporfin inhibits colon cancer progression independent of YAP1

Project description:Verteporfin (VP) inhibts colon cancer growth in vivo and in cell lines by inducing high molecular weight oligomerization of proteins. The antitumor effect of VP is independent of its YAP inhibitor activity. Tumor hypoxia contributes partly to antitumor effect of VP by impairing clearance of VP-induced high molecular weight aggregates.

2015-10-14 | PXD002793 | Pride

Effect of YAP-S127A overexpression on gene expression profile

Project description:To investigate the mechanism by which YAP regulates methionine metabolism, seven-week-old C57 mice were injected with AAV8-TBG-control or AAV8-TBG-YAP-S127A through tail vein. 8 weeks later, the primary hepatocytes from two groups were isolated for RNA sequence analysis.

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Gene expression profile in gastric cancer cells by Verteporfin (VP)

Project description:Verteporfin (trade name Visudyne) is a medication used as a photosensitizer for photodynamic therapy to eliminate the abnormal blood vessels in the eye associated with conditions such as the wet form of macular degeneration. Verteporfin accumulates in these abnormal blood vessels and, when stimulated by nonthermal red light with a wavelength of 689 nm[1] in the presence of oxygen, produces highly reactive short-lived singlet oxygen and other reactive oxygen radicals, resulting in local damage to the endothelium and blockage of the vessels. Recently, it was reported that VP has chemotherapeutic effect in cancer. To identify the gene expression profile in gastric cancer cells by VP, we performed microarray.

2017-12-26 | GSE77982 | GEO

Global gene expression profiling of mouse liver tissue samples after treated with various anti-HCV shRNAs

Project description:RNA interference (RNAi) is becoming routine in scientific discovery and treating human disease. However, its applications are hampered by unwanted effects. The RNA-Seq analyses from mouse liver samples expressing various anti-HCV shRNAs show that weak base-pairing in both seed and 3’ regions is required to achieve minimal off-targeting effect. We created nine shRNAs against the HCV genome, and transfected shRNA-expressing plasmids into mouse liver via a hydrodynamic tail vein infusion. A plasmid backbone lacking the shRNA sequence was used as a negative control. After 7 days, mouse liver RNAs were harvested and subject to RNA-Seq analysis (two biological repeats).

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Liver Clock Rescue

Project description:Circadian clocks drive 24-h rhythms of physiology and behavior. The circadian clock of hepatocytes has been shown to regulate glucose metabolism, and we were interested if rescuing liver clock function can reverse metabolic impairments in hyperphagic/obese Clock-D19 mutant mice. We compared transcripomte regulation in livers (at Zeitgeber time ZT10) of wild-type (C57BL/6J) and Clock-D19 mice and Clock-D19 mice with genetic rescue of liver clock function using hydrodynamic tail vein injection of a WT-CLOCK expression plasmid

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Myc and YAP roles in the control of the cell cycle [3T9 RNA-seq pharmacological treatment]

Project description:RNAseq analysis of YAP and Myc induced in quiescent and confluent 3T9 fibroblasts treated with Y27632 and Verteporfin

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Verteporfin targets CCR5 to inhibit TNBC growth and metastasis via YAP1

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2024-01-25 | GSE222052 | GEO

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