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An optimized protocol for isolation of hepatic leukocytes retrieved from murine and NASH liver biopsies

ABSTRACT: Immune dysregulation and inflammation by hepatic-resident leukocytes is considered a key step in disease progression of Non-alcoholic fatty liver disease (NAFLD) and Nonalcoholic steatohepatitis (NASH) toward cirrhosis and hepatocellular carcinoma (HCC). Here we provide a robust protocol for isolation and characterization of liver-resident immune cells from fine needle biopsies of rodent model and human. Various downstream applications can then be applied to gain an appreciation of the functional activity of liver-resident leukocyte populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE230131 | GEO | 2023/10/03

REPOSITORIES: GEO

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