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AoMedA has complex regulatory relationship with AoBrlA, AoAbaA and AoWetA in conidiation, trap formation, and secondary metabolism in nematode-trapping fungus Arthrobotrys oligospora

ABSTRACT: Transcriptomic analysis revealed that differentially expressed genes in the absence of AomedA and AoWetA were involved in mycelial growth, development process,conidiation, cell nucleus, trap formation, peroxisome, endocytosis and lipid metabolism.

ORGANISM(S): Orbilia oligospora

PROVIDER: GSE230299 | GEO | 2023/07/05

REPOSITORIES: GEO

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AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [gene expression]

Project description:Transcriptomic analysis revealed that differentially expressed genes in the absence of AoSte12 were involved in mycelial growth, development process,conidiation, cell nucleus and trap formation.

2023-01-29 | GSE213447 | GEO

TheArf‐GAPProteins AoAge1 and AoAge2 are Required for Mycelial Growth, Conidiation, Stress Responses, Trap Formation, and the Secondary Metabolism inthe Nematode-Trapping Fungus Arthrobotrys oligospora

Project description:ADP-ribosylation factors (Arfs) belong to the small GTPases superfamily and regulate mycelial development, endocytosis, and virulence in fungi. Nematode-trapping (NT) fungi can form unique infection structures (traps) to capture and kill free-living nematodes, thus play a potential role in the biocontrol of nematodes. Arthrobotrys oligospora is a representative species of the NT fungi. Here, the functions of two Arf-GAPs, Age1 (AoAge1) and Age2 (AoAge2) orthologue to S. cerevisiae were identified in A. oligospora by gene knockout and multi-phenotypic analysis. It was found that AoAge1 and AoAge2, especially AoAge2, play an important role in vegetative growth, conidiation, trap formation, DNA damage, endoplasmic reticulum stress, mitochondrial activity, endocytosis, heat shock stress, ROS level, and autophagy of A. oligospora. Importantly, our transcriptomic analysis (12 h) showed that nearly 62.7% of A. oligospora genes (11479) were directly or indirectly regulated by AoAge2, indicating that Arf-GAP plays a global role in the growth and development of A. oli gospora. Revealed by transcriptomic analysis differentially upregulated genes in the absence of Aoage2 were involved in autophagy and carbohydrate, amino acid and sphingolipid metabolism while downregulated genes were involved in longevity regulating pathway and carbohydrate, amino acid and secondary metabolites biosynthesis. In addition, metabolomic analysis showed that many compounds were markedly reduction in ΔAoage2 mutants strain. Our results highlighted that AoAge1 and AoAge2 play a crucial role in the asexual growth, development, differentiation, and lifestyle transition, especially, AoAge2 is indispensable for conidiation and trap morphogenesis of A. oligospora. These results demonstrated crucial roles for AoAge1 and AoAge2 in the fungal growth, environmental adaption and pathogenicity, which provided insights into the function of Arf-GAPs in A. oligospora and other fungi.

2024-01-26 | GSE253759 | GEO

The Arf‐GAP Proteins AoAge1 and AoAge2 are Required for Mycelial Growth, Conidiation, Stress Responses, Trap Formation, and the Secondary Metabolism in the Nematode-Trapping Fungus Arthrobotrys oligospora

2022-10-13 | GSE215079 | GEO

Aoatg11 and Aoatg33 are indispensable for mitophagy, and contribute to conidiation, the stress response, and pathogenicity in the nematode-trapping fungus Arthrobotrys oligospora

Project description:Mitophagy is one of the most important cellular processes to ensure mitochondrial quality control, which aims to transport damaged, dysfunctional, or excess mitochondria for degradation and reuse. Here, we determined the function of AoAtg11 and AoAtg33, two orthologous autophagy-related proteins involved in yeast mitophagy, in the typical nematode-trapping fungus Arthrobotrys oligospora . Deletion of Aoatg11 and Aoatg33 impairs mitophagy, mitochondrial morphology and activity, autophagy,cell apoptosis, reactive oxygen species levels, lipid droplet accumulation, and endocytosis. These combined effects resulted in slow vegetative growth; reduced conidiation, trap formation, cell nucleus, and extracellular protease activity; increased susceptibility to the stress response; and arthrobotrisin production in the Δ Aoatg11 and Δ Aoatg33 mutants, compared with the wild-type strain. In addition, the absence of Aoatg11 caused an endoplasmic reticulum stress response. Transcriptome analysis revealed that many differentially expressed genes in the Δ Aoatg11 mutants were involved in various important cellular processes, such as lipid metabolism, the TCA cycle, mitophagy, nitrogen metabolism, endocytosis, and the MAPK signaling pathway. In conclusion, our study revealed that Aoatg11 and Aoatg33 mediate autophagy and mitophagy in A. oligospora , and provides a basis for elucidating the links between mitophagy and fungal vegetative growth, conidiation, and pathogenicity.

2022-09-09 | GSE212735 | GEO

The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora.

Project description: Not available

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Role of Low-Affinity Calcium System Member Fig1 Homologous Proteins in Conidiation and Trap-Formation of Nematode-trapping Fungus Arthrobotrys oligospora.

Project description: Not available

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The Velvet Proteins VosA and VelB Play Different Roles in Conidiation, Trap Formation, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys oligospora.

Project description: Not available

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Cellular communication and fusion regulates cell fusion, trap morphogenesis, conidiation, and secondary metabolism in Arthrobotrys oligospora

Project description:In this study, we characterized Aoadv-1, Aoso, Aoham-6, and Aoham-5 of A. oligospora, homologous to those involved in cellular communication and fusion in the model fungus Neurospora crassa. The deletion of four genes resulted in the complete loss of cell fusion, and traps produced by mutants do not close to form mycelial rings but are still capable of capturing nematodes. Meanwhile, the absence of these genes inhibited aerial mycelial extension, slowed colony growth, and increased mycelial branching. In addition, the mutants showed reduced sporulation capacity, reduced tolerance to oxidative stress, increased sensitivity to SDS, and altered lipid droplet accumulation and autophagy. Furthermore, transcriptome analysis revealed that Aoadv-1 and Aoso were involved in plasma membrane fusion, transmembrane transport of multiple nutrients, signal transduction, response to oxidative stress, cell wall, lipid metabolism, and autophagy. In addition, metabolomic analysis showed that Aoadv-1 and Aoso were involved in biosynthesis of secondary metabolites. Our results revealed that Aoadv-1, Aoso, Aoham-6, and Aoham-5 regulate mycelial growth and trap morphogenesis through cell fusion, which provides a valuable reference for the molecular mechanisms of cellular communication regulating the mycelia development and trap morphogenesis in NT fungi.

2023-07-24 | GSE237909 | GEO

AMPK is involved in regulating the utilization of carbon sources, conidiation, pathogenicity, and stress response of the nematode-trapping fungus Arthrobotrys oligospora

Project description:Transcriptomic profile between the wild-type strain and ΔAosnf1 showed that differentially expressed genes were enriched on peroxisome,endocytosis, fatty acid degradation, and multi-lipid metabolism (sphingolipid, ether lipid, glycerolipid, and glycerophospholipid).

2022-06-07 | GSE205188 | GEO

Protein Kinase Ime2 Is Required for Mycelial Growth, Conidiation, Osmoregulation, and Pathogenicity in Nematode-Trapping Fungus Arthrobotrys oligospora.

Project description: Not available

| S-EPMC6971104 | biostudies-literature