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Simultaneous nanopore profiling of mRNA m6A and pseudouridine reveals translation coordination

ABSTRACT: N6-methyladenosine (m6A) and pseudouridine (Ψ) are the two most abundant modifications in mammalian mRNA, but the coordination of their biological functions remains poorly understood. We develop a machine learning-based nanopore direct RNA sequencing method (NanoSPA) that simultaneously analyzes m6A and Ψ in the human transcriptome. Applying NanoSPA to polysome profiling, we reveal opposing transcriptomic co-occurrence of m6A and Ψ and synergistic, hierarchical effects of m6A and Ψ on the polysome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE230936 | GEO | 2024/01/06

REPOSITORIES: GEO

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