Engineering oncogenic hotspot mutations on SF3B1 via CRISPR-directed PRECIS mutagenesis

ABSTRACT: To engineer novel SF3B1 mutant cell lines, we developed a methodology called PRECIS to install the pathogenic K700E mutation. We used this method to engineer CLL cell lines HG-3 and MEC-1 with the SF3B1 K700E mutation and then carried out RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE231387 | GEO | 2024/08/28

REPOSITORIES: GEO