Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis in RPMI-8402 cells after knockout of MYC

ABSTRACT: RNA-seq analysis was performed in a RPMI-8402 T-ALL cell stably expressing Cas9 after knockout of MYC

ORGANISM(S): Homo sapiens

PROVIDER: GSE231960 | GEO | 2023/08/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq analysis in RPMI-8402 cells after knockout of MYC and MYCN

Project description:RNA-seq analysis was performed in a RPMI-8402 T-ALL cell stably expressing Cas9 aafter knockout of both MYC and MYCN

2023-08-16 | GSE231962 | GEO

RNA-seq analysis in RPMI-8402 cells after knockout of MYCN

Project description:RNA-seq analysis was performed in a RPMI-8402 T-ALL cell stably expressing Cas9 after knockout of MYCN

2023-08-16 | GSE231959 | GEO

H3K27ac Hi-ChIP analysis in Jurkat and RPMI-8402 T-ALL cells

Project description:H3K27ac Hi-ChIP analysis was performed in Jurkat and RPMI-8402 stably expressing dCAR9-KRAB after treatment with DMSO (Control) or Doxycycline to induce sgRNA expression to block MYCN enhnacer region

2023-08-16 | GSE231957 | GEO

ATAC-seq analysis for Jurkat nd RPMI-8402 T-ALL cells

Project description:ATAC-seq analysis was performed in Jurkat and RPMI-8402 to analyze chromatin accessibility at steady state

2023-08-16 | GSE231955 | GEO

TAL1 complex in human T-cell acute lymphoblastic leukemia (RPMI-8402)

2012-07-09 | GSE39179 | GEO

TAL1 complex in human T-cell acute lymphoblastic leukemia (RPMI-8402)

Project description:The oncogenic transcription factor TAL1/SCL is aberrantly overexpressed in over 40% of cases of T-cell acute lymphoblastic leukemia (T-ALL), emphasizing the importance of the TAL1-regulated transcriptional program in the molecular pathogenesis of T-ALL. Here we identify regions occupied by TAL1 and its regulatory partners HEB, E2A, and GATA3 in a T-ALL cell line (RPMI-8402). Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), TCF12/HEB (Santa Cruz SC-357),TCF3/E2A (Santa Cruz SC-349X), and GATA3 (Santa Cruz SC-22206).

2012-07-09 | E-GEOD-39179 | biostudies-arrayexpress

Genome wide analysis of binding regions for MYC, MYCN and various histone modifications using Cut&Run

Project description:Cut&Run analysis was performed in RPMI-8402 cells to analyze binding regions of MYC, MYCN and various histone modifications in normal culture conditions

2023-08-16 | GSE231963 | GEO

Expression analysis of T-ALL cell lines TALL-1, RPMI-8402, SUPT11 and P12-ICHIKAWA after treatment with NVS-ZP7-3

Project description:Expression analysis of T-ALL cell lines TALL-1, RPMI-8402, SUPT11 and P12-ICHIKAWA after treatment with NVS-ZP7-3

| PRJNA475768 | ENA

Genome-scale CRISPR-Cas9 knockout screening for identification of genes which regulate CD38 expression in RPMI 8226 cells

Project description:Genome-scale CRISPR-Cas9 knockout screening for identification of genes which regulate CD38 expression in RPMI 8226 cells

| PRJNA643779 | ENA

Genome-scale CRISPR-Cas9 knockout screening for identification of genes which regulate CD38 expression in RPMI 8226 cells

Project description:We showed that interleukin-6 (IL-6) from bone marrow stromal cells downregulated CD38 expression in multiple myeloma (MM) cells. Then we performed a genome-scale CRISPR-Cas9 knockout screening to identify key molecules mediating IL-6-induced CD38 downregulation. Although IL-6 triggered downregulation of CD38 expression on the majority of RPMI 8226 cells, a small fraction maintained relatively high CD38 expression. We therefore sorted those top 5% cells with CD38-high expression and compared these cells with non-sorted control cells. The gene rank based on p-value identified Janus kinase (JAK) 1 and signal transducer-activator of transcription (STAT) 3 as highly positively enriched genes as potential mediators of IL-6-induced CD38 downregulation. We validated STAT3 and found CD38 expression was regulated by both STAT1 (positively) and STAT3 (negatively), and that inhibition of JAK-STAT3 pathway represents a novel therapeutic option to enhance CD38 expression and anti-CD38 monoclonal antibody-mediated MM cytotoxicity.

2020-07-03 | GSE153710 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data