Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA expression of mid-old fibroblasts co-cultured with young and mid-old fibroblasts

ABSTRACT: Examination of the difference in mRNA expression profile between mid-old fibroblasts co-cultured with mid-old fibroblasts and mid-old fibroblasts co-cultured with young fibroblasts

ORGANISM(S): Homo sapiens

PROVIDER: GSE232425 | GEO | 2023/05/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

mRNA expression of young, mid-old, and old fibroblasts

Project description:Examination of the difference in mRNA expression profile between young, mid-old, and old fibroblasts

2023-05-19 | GSE232427 | GEO

The gene expression of co-cultured mid old cells

Project description:Investigation of the rejuvenation effect to mid old cell, we co-cultured mid old cell with young cell or mid old cells. After 30days we harvest cells and extrect RNA then anlaysis the gene expression.

2022-04-30 | GSE201451 | GEO

The gene expression of co-cultured mid old cells

Project description:The gene expression of co-cultured mid old cells

| PRJNA831809 | ENA

The slit2 induces rejuveation in mid old cells.

Project description:The slit2 induces rejuveation in mid old cells such as reduces inflammation and inhibite cell cycle arrest.

2022-04-30 | GSE201457 | GEO

The slit2 induces rejuveation in mid old cells.

Project description:The slit2 induces rejuveation in mid old cells.

| PRJNA831835 | ENA

Small RNA next-generation sequencing of cardiac fibroblasts isolated from young and old mice

Project description:MiRNA expression profile was assessed in cardiac fibroblasts isolated from young (2 months old) and aged (16 months old) mice, by next generation sequencing of small RNA. Bioinformatic analysis of next-generation sequencing output demonstrated 88 differentially expressed miRNAs in aged mice - derived cardiac fibroblasts versus young mice-derived cells, of which two thirds were downregulated and one third were upregulated. The majority of downregulated miRNAs were mapped to chromosome 12, as part of Meg3-Mirg locus, while the remaining were spread on all chromosomes. Almost 90% of miRNA genes within Meg3-Mirg locus were expressed over a wide range in cardiac fibroblasts and had a decreasing trend in the aged group, the majority being significantly downregulated. This study provides evidence that aging is associated to significant alterations in miRNA expression profile in cardiac fibroblasts, a predominant feature being the downregulation of miRNAs within Meg3-Mirg locus.

2021-07-14 | GSE153214 | GEO

Expression data from skeletal muscle of young, old and old calorie restricted mice

Project description:We investigated age-related changes in the transcriptional profile of skeletal muscle in 5 month old (young) and 25 month old (old) C57BL/6NHsd mice using high density oligonucleotide arrays (22,690 transcripts probed). We identified 712 transcripts that are differentially expressed in young (5 month old) and old (25-month old) mouse skeletal muscle. Caloric restriction (CR) completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P<0.05), while CR significantly lowered expression levels for these genes as compared to control fed old mice (P<0.05). Age-related induction of p53-related genes was observed in multiple tissues, but was not observed in SOD2+/- and GPX4+/- mice, suggesting that oxidative stress does not mediate the observed age-related increase in expression. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. These observations support a role for p53-mediated apoptotic activity in mammalian aging. Keywords: aging, calorie restriction, muscle, p53

2006-12-01 | GSE6323 | GEO

Methylation profiling of directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL) to study age-associated changes.

Project description:This study comprises DNA methylation profiles of 9 directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL).

2024-01-01 | GSE247703 | GEO

Comparison of expression profile between fibroblasts taken from young (23 years old) and aged (70 years old) donors

Project description:Comparison of expression profile between fibroblasts taken from young (23 years old) and aged (70 years old) donors

| PRJNA482433 | ENA

Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts [ChIP-seq_nr6a1]

Project description:MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine Dicer-null adult mesenchymal stem cell lines, a fibroblast cell type. Loss of Dicer leads to de-repression of let-7 targets at levels that exceed 10-100 fold with increases in transcription. Direct and indirect targets of this miRNA belong to a mid-gestation embryonic program that encompasses known oncofetal genes as well as oncogenes not previously associated with an embryonic state. Surprisingly, this mid-gestation program represents a distinct period that occurs between the pluripotent state of the inner cell mass at embryonic day 3.5 and the induction of let-7, upon differentiation, at embryonic day 10.5. Within this mid-gestation program, we characterize the let-7 target Nr6a1, an embryonic transcriptional repressor that regulates gene expression in adult fibroblasts following miRNA loss. In total, let-7 is required for the continual suppression of embryonic gene expression in adult cells, a mechanism that may underlie its tumor suppressive function. Examination of genes directly bound by Flag-HA-NR6A1 in Dicer WT (Dicer f/f) adult mesenchymal stem cells (immortalized monoclonal lines of murine MSCs).

2013-04-10 | E-GEOD-44160 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data