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Single-cell and spatial transcriptomics profile of porcine jejunum and ileum containing Peyer's patches

ABSTRACT: To establish better understanding of cells found in jejunal and ileal Peyer's patches of pigs, we utilized single-cell RNA sequencing scRNA-seq and spatial transcriptomics to recover and analyze cells and spatial regions from sections of jejunum and ileum containing Peyer's patches. Cells identified via single-cell RNA sequencing included B, T/innate lymphoid cell, myeloid, epithelial, and stromal lineage cells. Spatial dots recovered via spatial transcriptomics belonged to regions including villi, crypts, interfollicular/parafollicular zones, follicles, and muscularis. Overall, results provide new information on regional localization and transcriptional profiles of cells in the pig small intestine.

ORGANISM(S): Sus scrofa

PROVIDER: GSE232605 | GEO | 2023/09/12

REPOSITORIES: GEO

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