Genomics

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MBP-FoxP3 pulldown-seq on naked DNA

ABSTRACT: To re-evaluate FoxP3 sequence specificity, we performed an unbiased pull-down of genomic DNA with recombinant FoxP3 protein. Use of genomic DNA, as opposed to synthetic DNA oligos, enables testing sequence specificity in the context of naturally existing repertoire of sequences. De novo motif analysis showed a strong enrichment of TnG repeats (n=2-5) by FoxP3 pull-down, using either MBP alone pull-down or input as a control.

ORGANISM(S): Mus musculus

PROVIDER: GSE232754 | GEO | 2023/10/09

REPOSITORIES: GEO

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