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In vivo clonal tracking of human skeletal stem cells with single cell RNA-sequencing readout

ABSTRACT: 10X Genomics single cell RNA-sequencing of freshly isolated human skeletal stem cells from the growth plate or periosteum of 17 weeks gestational age fetal bone tissue were collected. The goal of this work was to trace single cell dynamics of human skeletal stem cells in an in vivo setting. Single barcoded human skeletal stem cells were tracked in the graft tissue they formed after xenotransplanation into mice. Single cell analysis assessed tissue composition and was overlayed with barcode readouts to find contributions of individual clones. Differences in stem cell properties and lineage dynamics were assessed between growth plate and periosteal human skeletal stem cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE233188 | GEO | 2025/02/25

REPOSITORIES: GEO

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