Differential expression of genes in pancreatic islet of C57Bl/6J on high fat diet
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE23325 | GEO | 2011/11/20
SECONDARY ACCESSION(S): PRJNA131225
REPOSITORIES: GEO
Similar Datasets
Project description:Male C57Bl/6J mice were fed 45%kcal fat diet (HF) or regular rodent chow (NC) from 4 weeks to 16 weeks of age. Gene expression was compared between RNA obtained from pancreatic islets of HF fed mice and NC mice. RNA samples from 4 NC group and 4 HF groups were analyzed using GeneChip Mouse Expression Arrays MOE 430v2 (Affymetrix).
2011-11-20 | E-GEOD-23325 | biostudies-arrayexpress
Project description:Impaired myocardial contractile function is a hallmark of heart failure (HF) which may present under resting conditions and/or during physiological stress. Previous studies reported that high fat feeding in HF is associated with improved myocardial contractile function at baseline. Our goal was to determine whether myocardial function is compromised in response to physiological stress and to evaluate the global gene expression profile of rats fed high dietary fat following infarction. Male Wistar rats underwent ligation or sham surgery and were fed normal (10% kcal fat) (SHAM+NC, HF+NC) or high fat (60% kcal saturated fat) (SHAM+SAT, HF+SAT) for 8 weeks. Myocardial contractile function was assessed using a Millar pressure-volume (PV) conductance catheter at baseline, during inferior vena caval occlusions and dobutamine (DOB) stress. Steady state indices of systolic function, left ventricular (LV)+dP/dtmax, stroke work and maximal power were increased in HF+SAT vs HF+NC; HF+NC were reduced vs SHAM+NC. Preload-recruitable measures of contractility [end systolic PV relationship, maximal elastance, preload recruitable SW and peak+dP/dtmax to end diastolic volume] were decreased in HF+NC but not HF+SAT. β-adrenergic responsiveness (delta-LV+dP/dtmax and delta-cardiac output DOB 0-10 µg•kg-1•min-1) was reduced in HF, but high fat feeding did not further impact contractile reserve in HF. Contractile reserve was reduced by high fat in SHAM+SAT. Microarray gene expression analysis reveals the majority of significantly altered pathways identified to contain multiple gene targets correspond to cell signaling pathways and energy metabolism. These findings suggest that high saturated fat improves myocardial function at rest and during physiological stress in infarcted hearts, but may negatively impact contractile reserve under non-pathological conditions. Furthermore, high fat feeding-induced alterations in gene expression related to energy metabolism and specific signaling pathways reveal promising targets through which high saturated fat potentially mediates cardioprotection in heart failure/LV dysfunction. Comparison of gene expression in heart failure or sham surgery hearts exposed to saturated or normal diets. Male Wistar rats (300-350g) were maintained on a reverse light-dark cycle and all procedures were done 3-6 hours into the dark phase cycle to synchronize with the normal active state of the rodents. Rats were randomly assigned to receive either a sham-operation (SH) or coronary ligation to induce cardiac dysfunction (HF). Heart failure was induced by ligating the left main coronary artery. Following surgery, rats were immediately fed either a normal rodent chow (NC) or a high saturated fat chow (SAT) with 60% caloric content derived from fat (25% palmitic, 33% stearic, 33% oleic acid, Research Diets).
2010-09-01 | E-GEOD-21021 | biostudies-arrayexpress
Project description:This study sought to interrogate the effects of lipids and lipid metabolites on the hepatic proteome. Protein expression in high-fat diet (HFD) mouse livers vs. livers of normal chow fed (NC) mice were investigated using multiplexed quantitative LC-MS/MS (TMT labeling). This experiment contains additional replicates for normal chow and mice on high-fat diet for 16 weeks.
2019-07-08 | PXD013815 | Pride
Project description:In order to identify gene expression patterns that vary with diet, genetics, and their interaction, we performed expression profiling by RNA-Seq across four different tissues (liver, gonadal fat, soleus muscle, and pancreas) of TALLYHO/Jng (TH) and C57BL/6J (B6) male (M) and female (F) mice, fed either a chow or high fat (HF) diet at 5 weeks and 20 weeks of age.
2023-11-30 | GSE234011 | GEO
Project description:Impaired myocardial contractile function is a hallmark of heart failure (HF) which may present under resting conditions and/or during physiological stress. Previous studies reported that high fat feeding in HF is associated with improved myocardial contractile function at baseline. Our goal was to determine whether myocardial function is compromised in response to physiological stress and to evaluate the global gene expression profile of rats fed high dietary fat following infarction. Male Wistar rats underwent ligation or sham surgery and were fed normal (10% kcal fat) (SHAM+NC, HF+NC) or high fat (60% kcal saturated fat) (SHAM+SAT, HF+SAT) for 8 weeks. Myocardial contractile function was assessed using a Millar pressure-volume (PV) conductance catheter at baseline, during inferior vena caval occlusions and dobutamine (DOB) stress. Steady state indices of systolic function, left ventricular (LV)+dP/dtmax, stroke work and maximal power were increased in HF+SAT vs HF+NC; HF+NC were reduced vs SHAM+NC. Preload-recruitable measures of contractility [end systolic PV relationship, maximal elastance, preload recruitable SW and peak+dP/dtmax to end diastolic volume] were decreased in HF+NC but not HF+SAT. β-adrenergic responsiveness (delta-LV+dP/dtmax and delta-cardiac output DOB 0-10 µg•kg-1•min-1) was reduced in HF, but high fat feeding did not further impact contractile reserve in HF. Contractile reserve was reduced by high fat in SHAM+SAT. Microarray gene expression analysis reveals the majority of significantly altered pathways identified to contain multiple gene targets correspond to cell signaling pathways and energy metabolism. These findings suggest that high saturated fat improves myocardial function at rest and during physiological stress in infarcted hearts, but may negatively impact contractile reserve under non-pathological conditions. Furthermore, high fat feeding-induced alterations in gene expression related to energy metabolism and specific signaling pathways reveal promising targets through which high saturated fat potentially mediates cardioprotection in heart failure/LV dysfunction.
2010-09-01 | GSE21021 | GEO
Project description:Inflammatory monocyte (MC) subset differentiation is a major feature in tissue inflammatory and atherosclerosis. The underlying molecular mechanism remains unclear. This study aims to explore molecule targets and signaling which determinate immunological features in MC subsets. ApoE-/- mice were fed a normal chow diet and switched to a high-fat (HF) diet at age 8 weeks and maintained on a HF diet for 12-14 weeks. The control C57BL/6 mice were fed a normal chow diet throughout. Blood Ly6Chigh and Ly6Clow MC subsets from control and ApoE-/- mice were isolated by flow cytometry sorting and subjected for bulk high-throughput RNA-sequencing.
2022-01-11 | GSE189097 | GEO
Project description:Purpose: To investigate the effects of weight cycling on the metabolic homeostasis Methods: C57BL/6 mice were fed with high fat diet (HF) (60% kcal fat; Research Diets Inc., New Brunswick, NJ) or low fat diet (LF) (10% kcal fat; Research Diets Inc.) at 6-week-old. Nine weeks after in HF, a part of mice was switched to LF for one week followed by one week HF as one cycle, and the mice under this one week diet switch protocol for 10 cycles were named HF-LF cycled mice, while the left part of mice under continuously HF-feeding were named HF mice. The mice kept in the continuously LF-feeding were named LF mice Results: We identified many changed genes in the eWAT by RNA-sequencing. Conclusions: This finding provides a signaling in eWAT upon weight cycling
2021-09-30 | GSE134336 | GEO
Project description:Hypercholesterolemai is a major contributor to atherosclerosis development. To assess the effects of hypercholesterolemia on the transcriptional profiling in foam cells, mice were fed regular chow, or WD for 2 or 14 weeks prior to sacrifice. We used microarrays for broad analysis of gene expression in the 3 experimental groups. Each group included 5 mice. ApoE-/- mice were fed regular mouse chow to the age of 22 weeks (CHOW) or a western-type diet for 2 weeks between weeks 22 and 24 (2w-WD) or for 14 weeks between weeks 8 and 22 (14w-WD). Foam cells in cross-sections of the aourtic sinus were isolated by laser-capsure microdissection (n=5 per group). RNA was amplified by in vitro transcription, biotinylated, and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays.
2015-07-09 | E-GEOD-70619 | biostudies-arrayexpress
Project description:The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. Briefly, 7-week-old male C57BL/6J mice purchased from Charles River Laboratories Japan (Yokohama) were divided into the following five groups. The normal diet group (ND group) was fed D12450B (10 kcal% fat, Research Diets, New Brunswick, NJ, USA). The high-fat diet group (HF group) was fed D12492 (60 kcal% fat, Research Diets, New Brunswick, NJ, USA). The caffeinated coffee group (HFCC group) was fed a high-fat diet containing 2% caffeinated freeze-dried coffee. The decaffeinated coffee group (HFDC group) was fed a high-fat diet containing 2% decaffeinated freeze-dried coffee. The green unroasted coffee group (HFGC group) was fed a high-fat diet containing 2% unroasted caffeinated freeze-dried coffee. The mice had ad libitum access to their diets and drinking water. After 9 weeks, mice were sacrificed and the livers were subjected to the Affymrtix DNA microarray experiment.
2013-12-10 | E-GEOD-53131 | biostudies-arrayexpress
Project description:We propose comparing liver gene expression of WT and female ERKO mice early in the high-fat feeding period to animals fed a regular chow diet. Analyzing liver tissue before the fatty liver disease phenotype becomes severe will allow identification of target genes which may be causal. Comparison of regular chow fed WT animals to high fat fed WT animals will allow for identification of hepatic genes up-regulated in response to high fat feeding. Comparison of regular chow fed WT animals to regular chow fed ERKO animals will help clarify hepatic gene expression patterns that may be implicated in increased susceptibility to weight gain and glucose intolerance. Comparison of high fat fed WT animals to high fat fed ERKO animals will provide insight into genes that could be implicated in leading to increased fat accumulation in the liver over time during high fat feeding. Finally, comparison of regular chow fed ERKO animals to high fat fed ERKO animals will help identify genes that may be contributing to increased liver fat accumulation in response to high fat feeding in these animals.
2017-03-22 | GSE95283 | GEO