Mouse Thyroid Nanopore RNAseq
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE233457 | GEO | 2023/05/26
REPOSITORIES: GEO
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Project description:Thyroglobulin protein is the precursor of thyroid hormones, which are essential for growth, development and control of metabolism in vertebrates. Hormone synthesis from thyroglobulin (TG) occurs in the thyroid gland via the iodination and subsequent coupling of pairs of tyrosine amino acids, whose positions in TG have not been clearly identified. Tyrosine proximity within TG is thought to enable the coupling reactions but the lack of a three-dimensional structure of TG has prevented mechanistic understanding. Here we determined the structure of full-length human thyroglobulin at ~3.4 Å resolution by cryo-electron microscopy (cryo-EM). To enable perturbation experiments we expressed human TG in human HEK cells and showed it to be active in hormone production. We identified all hormogenic tyrosine pairs in the structure and verified them via site-directed mutagenesis and in vitro hormone production assays. Analysis revealed that proximity, flexibility and solvent exposure of the tyrosines are key characteristics of the hormogenic sites. To support the validity of our insights, we engineered the small bacterial protein MBP (maltose binding protein) to produce thyroid hormones with similar efficiency as TG. Finally, our study provides an essential framework to further understand the production and regulation of thyroid hormones, including in thyroid diseases.
2020-02-06 | PXD014821 | Pride
Project description:Thyroid hormones T3 and T4 play a crucial role in the regulation of vertebrate metabolism. The thyroglobulin (Tg) protein is key to thyroid hormone synthesis, and its structure is conserved among vertebrates. TgG is delivered through the secretory pathway to the lumen of the thyroid gland, where it is iodinated at specific tyrosine sites to form mono- or di-iodotyrosine, which combine to produce T3 and T4, respectively. The formation of these hormones depends on the precise 3D structure of thyroglobulin, which has remained unknown despite decades of research on this protein. Here, we present the cryo-electron microscopy structure of human thyroglobulin, to a global resolution of 3.2 Å. Our results offer structural insight into thyroid hormonogenesis and provide a framework to understand hundreds of clinically relevant mutations. The structure of hTg contributes to a better understanding and potentially improved treatment of thyroid hypothyroidism, thyroid cancer and other Tg disorders.
2022-02-24 | PXD018452 | Pride
Project description:The Tthyroglobulin (Tg) protein is the essential fundament toto thyroid hormone synthesis, a key player playing a vital role in the regulation of metabolism, development and growth and serving as intraglandular iodine storage. Its structure is conserved among vertebrates. Tg is delivered through the secretory pathway of the thyroid follicular unit to the central colloid depository, where it is iodinated at specific tyrosine sites to form mono- or diiodotyrosine, which combine to produce triiodothyronine (T3) and thyroxine (T4), respectively. Synthesis of these hormones depends on the precise 3D structure, iodination capacity and post-translational modification of Tg. The Tg structure is overall conserved among vertebrates and , which and structural information has long been remained unknown missing despite decades of research until recently structural information has been made available on human thyroglobulin (hTg) originating from recombinant hTg and endogenous hTg from patients with goitres. Here, we present the cryo-electron microscopy structure of native and fully functional human thyroglobulin (hTg) originating from healthy human thyroid glands, to a global resolution of 3.2 Å. We used LC-MS experiments to The structure provides detailed information on the location of the hTg hormonogenic sites by revealing native iodination, and reveals the position as well as the role of many of its glycosylation sites and other post-translational modifications. Our results offer structural insights into thyroid hormonogenesis of natively functional hTg and provide a fundamental understanding of clinically relevant hTg mutations, which can improve treatment of thyroid diseases.
2022-02-17 | PXD029013 | Pride
Project description:The large secretory glycoprotein, thyroglobulin, is the primary translation product of thyroid follicular cells. This difficult-to-fold protein is readily susceptible to structural alterations that render the misfolded thyroglobulin unable to be exported from the endoplasmic reticulum (ER) a known cause of congenital hypothyroidism, with severe, chronic thyrocyte ER stress. Nevertheless, patients with this disease commonly grow a goiter indicative of thyroid cell survival and adaptation. To model this, we have treated PCCl3 thyrocytes with continuous exposure to tunicamcyin (causing an ER stress that can be specifically attributed to thyroglobulin misfolding). In response, PCCl3 cells escape by downregulating expression of Mfsd2a (the tunicamycin transporter). By contrast, following CRISPR/Cas9-mediated deletion of Mfsd2a, PCCl3 cells cannot escape the continuous, chronic effects of high-dose tunicamycin (as demonstrated by persistent accumulation of unglycosylated thyroglobulin); nevertheless the thyrocytes live and grow. A comprehensive proteomic analysis of these cells adapted to chronic ER protein misfolding reveals many hundreds of up-regulated proteins, supporting stimulation of ER chaperones, oxidoreductases, and stress responses as well as lipid biosynthesis pathways. Further, we noted: a) increased phospho-AMP-kinase-B (suggesting upregulated AMPK activity) and decreased phospho-S6 and protein translation (suggesting decreased mTOR activity), consistent with conserved cell survival/adaptation pathways; and b) a suggestion of less differentiated thyrocyte phenotype with decreased PAX8, FOXE1, and TPO protein, as well as a significant decrease of thyroglobulin mRNA levels.
2020-02-25 | MSV000085006 | MassIVE
Project description:The RET/PTC3 (RP3) fusion gene is the most frequent mutation found in radiation-induced papillary thyroid cancers (PTC). Several studies suggest that the RET/PTC rearrangement is an initiating event in tumorigenesis. E7 is an oncoprotein derived from the Human Papilllomavirus 16 (HPV16) responsible for most cervical carcinoma in women. We studied here the sequence of events leading to thyroid cancer in Tg-RP3 and Tg-E7 mice expressing the transgene exclusively in the thyroid under the control of thyroglobulin (Tg) promoter. Both transgenic mice develop thyroid hyperplasia followed by solid differentiated carcinoma in older animals. To understand the different steps leading to carcinoma, we analyzed thyroid gene expression in both strains at different ages (2, 6, 10 months) by microarray technology. Important biological processes were differentially regulated in the two tumor types. In E7 thyroids cell cycle was the most upregulated process; observation consistent with the huge size of these tumors. In RP3 thyroids immunity was the most significantly regulated process, as previously observed in microarray data on human PTC. Interestingly, other human PTC characteristics were also observed in RP3 but not in E7 mouse tumors: similar regulation of several human PTC markers, upregulation of many EGF-like growth factors and finally significant regulation of angiogenesis and extracellular matrix remodeling-related genes. In summary we showed that RP3 contrary to E7 mouse tumors share several important genotypic characteristics with human PTC, observation reinforcing the validity of this model to study human thyroid tumorigenesis. Keywords: time course, genetic modification, disease state, cancer
2008-07-18 | GSE10743 | GEO
Project description:Thyroglobulin (Tg) is a secreted iodoglycoprotein serving as the precursor for T3 and T4 hormones. Many characterized Tg gene mutations produce secretion-defective variants resulting in congenital hypothyroidism (CH). Tg processing and secretion is controlled by extensive interactions with chaperones, trafficking, and degradation proteins comprising the secretory proteostasis network. While dependencies on individual pro-teostasis network components are known, the integration of proteostasis pathways mediating Tg protein quality control and the molecular basis of mutant Tg misprocessing remain poorly understood. We employ a multiplexed quantitative affinity purification–mass spectrometry approach to define the Tg proteostasis interactome and changes between WT and several CH-variants. Mutant Tg processing is associated with common imbalances in proteostasis engagement including increased chaperoning, oxidative folding, and routing towards ER-associated degradation components, yet variants are inefficiently degraded. Furthermore, we reveal mutation-specific changes in engagement with N-glycosylation components, suggesting distinct requirements of one Tg variant on dual engagement of both oligosaccharyltransferase complex isoforms for degradation. Modulating dysregulated proteostasis components and pathways may serve as a therapeutic strategy to restore Tg secretion and thyroid hormone biosynthesis.
2020-11-20 | PXD019427 | Pride
Project description:Notch signaling between neighboring cells vitally contributes to specifying their identity and function, during development and in the adult. Here, we demonstrate that selective blockade of the Notch ligands JAG1 and JAG2 disrupts thermoregulation in adults by decreasing circulating thyroid hormones and thyroid function. By exploiting single-cell methods to carefully evaluate the thyroid, an understudied apex endocrine organ, we discovered unexpected thyrocyte heterogeneity, marked by three distinct thyrocyte subtypes with different Notch activation patterns.
2023-10-23 | GSE200267 | GEO
Project description:We developed a novel culture system to obtain multilineage undifferentiated stem/progenitor cells from normal human thyroid tissues. This seems to be achieved by direct reprogramming of thyroid follicular cells. The objective of the study was to reveal gene expression profile of the obtained cells compared to primary thyrocytes. After enzymatic digestion, primary thyrocytes, expressing thyroglobulin and cytokeratin-18, were cultured in a serum-free medium called SAGM containing insulin and EGF. Although the vast majority of cells died, a small proportion (~0.5%) survived and proliferated. During initial cell expansion, thyroglobulin/cytokeratin-18 expression was gradually declined, suggesting that those cells are derived from thyroid follicular cells or at least thyroid-committed cells. The SAGM-grown cells did not express any thyroid-specific genes. However, after four-week incubation with FBS and TSH, cytokeratin-18, thyroglobulin, TSH receptor, PAX8 and TTF1 expressions re-emerged. Moreover, surprisingly, the cells were capable of differentiating into neuronal or adipogenic lineage depending on differentiating conditions.
2011-04-15 | GSE24553 | GEO
Project description:We developed a novel culture system to obtain multilineage undifferentiated stem/progenitor cells from normal human thyroid tissues. This seems to be achieved by direct reprogramming of thyroid follicular cells. The objective of the study was to reveal gene expression profile of the obtained cells compared to primary thyrocytes. After enzymatic digestion, primary thyrocytes, expressing thyroglobulin and cytokeratin-18, were cultured in a serum-free medium called SAGM containing insulin and EGF. Although the vast majority of cells died, a small proportion (~0.5%) survived and proliferated. During initial cell expansion, thyroglobulin/cytokeratin-18 expression was gradually declined, suggesting that those cells are derived from thyroid follicular cells or at least thyroid-committed cells. The SAGM-grown cells did not express any thyroid-specific genes. However, after four-week incubation with FBS and TSH, cytokeratin-18, thyroglobulin, TSH receptor, PAX8 and TTF1 expressions re-emerged. Moreover, surprisingly, the cells were capable of differentiating into neuronal or adipogenic lineage depending on differentiating conditions. Total RNAs were extracted from PT from three different samples (PT0808, PT0811 and PT0812) and corresponding SAGM-grown lines and subjected to Affymetrix GeneChip Human Genome U133 Plus 2.0 microarray analysis
2011-04-15 | E-GEOD-24553 | biostudies-arrayexpress
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