Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomics revealed sex differences in human pulmonary microvascular endothelial cells in normoxia and hypoxia

ABSTRACT: To investigate sex differences at the transcriptome level in human pulmonary microvascular endothelial cells (HPMECs) from healthy male and female donors basally (in normoxia) and in hypoxic conditions. RNA-seq was performed on male (n=3) and female (n=4) HPMECs that were cultured in conditions of physiological shear stress (PMID: 36730645) in normoxia (21% O2) or in hypoxia (1% O2) for either 24 or 48 hours.

ORGANISM(S): Homo sapiens

PROVIDER: GSE233547 | GEO | 2023/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)

Project description:Endothelial cells, and many other types of cells too, physiologically reside in low O2 environments (~ 2-13% O2 or 15-60 mmHg pO2; [PCN]) relevantly to atmospheric O2 (~ 21% or 160 mmHg pO2 at sea level) in vivo. Such PCN is critical for endothelial functions. The majority of our current knowledge regarding the cellular and signaling mechanisms governing endothelial functions, however, is built on endothelial models established under atmospheric O2 (~21% O2). Herein, we comapred the transcriptional profiles between HUVE and HUAE cells cultured and expanded under PCN (3% oxygen) and standard culture normoxia (21% O2).

2013-08-30 | GSE49958 | GEO

Transcriptomic profiling of wild-type and Bmal1-/- myoblasts under normoxia or actue hypoxia conditions

Project description:Purpose: The goal of this study is to investigate the role of interplay between circadian clock and oxygen-sensing pathways in determining myogenic progenitor cell fate. Methods: Total RNAs were extracted from wild-type and Bmal1-/- myoblasts following exposure to normoxia (21% O2) or hypoxia (1% O2) for 6 hours, and subjected to RNA-sequencing. Results: There were significantly up-regulated (443 in normoxia versus 477 in hypoxia) and down-regulated (745 in normoxia versus 796 in hypoxia) genes in Bmal1-/- cells compared to wild-type, with a large degree of overlap between hypoxia and normoxia, although the fold change of differential gene expression was generally greater under hypoxia versus normoxia. Conclusion: Loss of Bmal1 in myoblasts leads to a premature differentiation-prone transcriptome, which was exaggerated following exposure to hypoxia.

2022-07-01 | GSE184741 | GEO

Expression data from adult Drosophila females [normoxia]

2014-02-04 | GSE46358 | GEO

Gene expression signature of WT siblings and hif1α mutants in normoxia and after hypoxia chamber incubation

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes with differential expression in WT and hif1α mutants and consequent different response to normoxia or hypoxia conditions. Two different conditions were considered: 50 hpf mutants and WT in normoxia, 50 hpf mutants and WT after hypoxia chamber treatment (2 hours after 3% O2)

2017-05-01 | GSE89117 | GEO

Proteomics of human lens epithelial cells under normoxia and hypoxia

Project description:The HLE cells were seeded in three 10cm Petri dishes overnight and treated for 24h under hypoxia(1% O2) and normoxia conditions respectively. The treated cells were collected as samples, and subjected to Tandem Mass Tag (TMT) Labeling and High pH Reversed-Phase Peptide Fractionation.

2023-04-10 | PXD041436 |

Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)

2013-08-30 | E-GEOD-49958 | biostudies-arrayexpress

Expression data from adult Drosophila females [normoxia and IH]

Project description:High-fat diet (HFD) in normoxia causes a worsened phenotype in adult female flies compared to regular diet (RD). Intermittent hypoxia (IH) causes an opposite phenotype both when flies are on RD in IH compared to normoxia and even more dramatically when on HFD in IH compared to HFD in normoxia. We used microarrays to examine the transcriptional changes occurring both on a HFD compared to RD in normoxia, and when IH is introduced.

2015-05-30 | GSE47166 | GEO

Expression data from adult Drosophila females [normoxia]

Project description:High-fat diet (HFD) in normoxia causes a worsened phenotype in adult female flies compared to regular diet (RD). We used microarrays to examine the transcriptional changes occuring on a HFD compared to RD in normoxia. Adult female Drosophila were collected at d3-5 and placed on RD or HFD and in normoxia for 1 week. Following this week,flies were immediately frozen on dry ice, RNA was extracted and hybridized on Affymetrix microarrays.

2014-02-04 | E-GEOD-46358 | biostudies-arrayexpress

Transcriptomic profiles reveal differences between the right and left ventricle (RV and LV) in both normoxia and hypoxia

Project description:We performed RNA-seq to analyze gene expression in rat cardiac tissue from the left and right ventricle for animals in a) normoxia and b) exposed to hypoxia (10% O2) for 2 weeks

2020-02-03 | GSE133402 | GEO

CDK8 ChIP-seq from HCT116 cells in normoxia and hypoxia

Project description:To test if CDK8 acts directly at HIF1A target genes, we performed ChIP-seq experiments in HCT116 cells under normoxic and hypoxic conditions. ChIP-seq for CDK8 versus Input under normoxia and 24hrs hypoxia (1% O2).

2013-03-01 | E-GEOD-38258 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data