Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells I

ABSTRACT: To investigate the function of Hand1 and Foxf1 in the regulation of Cardiac differentiation, we established E14 cell lines in which each target gene has been knocked out by sgRNA.

ORGANISM(S): Mus musculus

PROVIDER: GSE233586 | GEO | 2025/12/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells II

Project description:To investigate the function of Hand1 and Foxf1 in the regulation of Cardiac differentiation, we established E14 cell lines in which each target gene has been knocked out by sgRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of these cell lines at different time points during the CM differentiation process.

2025-12-08 | GSE245711 | GEO

Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells I

Project description:Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells I

| PRJNA976697 | ENA

Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells II

Project description:Effect of depletion of Hand1 and Foxf1 on gene expression during CM differentiation of E14 embryonic stem cells II

| PRJNA1029605 | ENA

Functional CRISPR Screen Identifies AP1-associated Enhancer regulating FOXF1 to modulate Oncogene-Induced Senescence

Project description:We carried out CRISPR-Cas9 functional screen focused on AP-1 bound enhancers that are activated upon oncogenic stress. The screen discovered Enh.AP1.OIS1 - an enhancer bound by AP1 that is required for activation of oncogene-induced senescence (OIS) response. We applied RNA-seq analysis to RASG12V-activated BJ cells transduced with either sgRNAs targeting AP-1 bound enhancer (sgRNA-69 or sgRNA71), or sgRNA targeting the target gene FOXF1 or non-targeting control sgRNA (sgNT)

2018-07-15 | GSE112458 | GEO

HAND1 Ubiquitination and Phosphorylation sites

Project description:pcDNA3-HAND1-his was transfected into HEK293T cells and harvested after 48 hours. IMAC was carried out under denaturing conditions to enrich for HAND1,and samples were loaded onto SDS-PAGE gel with HAND1 gel band excised and submitted to LC-MS/MS.

2020-12-18 | PXD022407 | Pride

E11.5 Hand1 myocardial knockout RNA-Seq

Project description:Aims: To examine the role of the basic Helix-loop-Helix (bHLH) transcription factor HAND1 in embryonic and adult myocardium. Methods and Results: Hand1 is expressed within the cardiomyocytes of the left ventricle (LV) and myocardial cuff between embryonic days (E) 9.5-13.5. Hand gene dosage plays an important role in ventricular morphology and the contribution of Hand1 to congenital heart defects requires further interrogation. Conditional ablation of Hand1 was carried out using either Nkx2.5 knockin Cre (Nkx2.5Cre) or a-myosin heavy chain Cre (aMhc-Cre) driver. Interrogation of transcriptome data via Ingenuity Pathway Analysis (IPA) reveals several gene regulatory pathways disrupted including translation and cardiac hypertrophy-related pathways. Embryo and adult hearts were subjected to histological, functional and molecular analyses. Myocardial deletion of Hand1 results in morphological defects that include cardiac conduction system defects, survivable interventricular septal defects (VSDs), and abnormal LV papillary muscles (PM). Resulting Hand1 conditional mutants are born at Mendelian frequencies; but the morphological alterations acquired during cardiac development result in, the mice developing diastolic heart failure. Conclusions: Collectively, these data reveal that Hand1 contributes to the morphogenic patterning and maturation of cardiomyocytes during embryogenesis and although survivable, indicate a role for Hand1 conduction system and papillary morphogenesis.

2019-06-01 | GSE128571 | GEO

Foxf1 enchances pulmonary inflammation and mastocytosis

Project description:The Forkhead Box f1 (Foxf1) transcriptional factor (previously known as HFH-8 or Freac-1) is expressed in endothelial and smooth muscle cells in the embryonic and adult lung. To assess effects of Foxf1 during lung injury, we used CCl4 injury model. Foxf1+/- mice developed severe airway obstruction and bronchial edema, associated with increased numbers of pulmonary mast cells and increased mast cell degranulation following injury. Pulmonary inflammation in Foxf1+/- mice was associated with diminished expression of Foxf1, increased mast cell tryptase and increased expression of CXCL12, the latter being essential for mast cell migration and chemotaxis. Foxf1 haploinsufficiency caused pulmonary mastocytosis and enhanced pulmonary inflammation following chemically-induced lung injury, indicating an important role for Foxf1 in the pathogenesis of pulmonary inflammatory responses. Keywords: Influence of genetic modification on the pulmonary inflamation Foxf1+/- mice in which the Foxf1 allele was disrupted by an in-frame insertion of a nuclear localizing -galactosidase (-Gal) gene were bred for ten generations into the Black Swiss mouse genetic background. Carbon tetrachloride (CCl4; Sigma, St Louis, MO) was dissolved in mineral oil at a 1:20 ratio v/v and a single intraperitoneal (i.p.) injection of CCl4 (0.5 l of CCl4/ 1g of body weight) was administered to male Foxf1+/- mice or their wild type (WT) littermates as described.

2008-09-04 | E-GEOD-11112 | biostudies-arrayexpress

Foxf1 enchances pulmonary inflammation and mastocytosis

2008-09-04 | GSE11112 | GEO

Mouse embryonic lung FOXF1 ChIP-seq analysis

Project description:FOXF1 targets in embryonic day 18.5 mouse lungs were determined using FOXF1 immunoprecipitation followed by sequencing. FOXF1 binding sites in two biological replicates of pooled E18.5 wildtype mouse lungs (n=3) were to a 2% input control sample.

2016-09-01 | E-GEOD-77951 | biostudies-arrayexpress

Foxf1+/- mouse lung gene expression analysis

Project description:Analysis of genes dysregulated due to haploinsufficiency of Foxf1 in postnatal day 0.5 mouse lungs Total RNA obtained from six Foxf1+/- mouse lungs compared to six wildtype littermate mouse lungs

2014-03-01 | E-GEOD-54128 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data