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Genes regulated by SDC1

ABSTRACT: We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of MDA-MB-231 cells transfected with SDC1-siRNA against negative control siRNA-transfected cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE23362 | GEO | 2013/08/01

SECONDARY ACCESSION(S): PRJNA131249

REPOSITORIES: GEO

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Genes regulated by SDC1

Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of MDA-MB-231 cells transfected with SDC1-siRNA against negative control siRNA-transfected cells. MDA-MB-231 cells were transfected with SDC1-siRNA or negative control siRNA. Subsequently, total RNA was collected and processed for analysis using Affymetrix microarrays. Three independent replicates were prepared for each comparison group.

2013-08-01 | E-GEOD-23362 | biostudies-arrayexpress

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Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of Caco-2 cells transfected with SDC1-siRNA against negative control siRNA-transfected cells.

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Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of HS3ST2-transfected and control vector-transfected MDA-MB-231 cells.

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Genes regulated by HS3ST2

Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of HS3ST2-transfected and control vector-transfected MDA-MB-231 cells. MDA-MB-231 cells were transfected with HS3ST2- or control-vector. Subsequently, total RNA was collected and processed for analysis using Affymetrix microarrays. Three independent replicates were prepared for each comparison group.

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Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of miR-145-overexpressing MDA-MB-231 cells against negative control miRNA precursor-transfected cells. MDA-MB-231 cells were transfected with pre-miR-145 or a negative control pre-miRNA, and subsequently total RNA was collected and processed for analysis using Affymetrix microarrays. Three independent replicates were prepared for each comparison group.

2010-09-07 | E-GEOD-19737 | biostudies-arrayexpress

Homo sapiens

Project description:Hypoxic MDA-MB-231 U133 Plus 2.0 array

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Global gene expression analysis of CDCP1, KDM3B, and SDC1 knockdown in human marrow stromal cells

Project description:To elucidate functions of CDCP1, KDM3B and SDC1 molecules in HS5 human marrow stromal cells, global gene expression profiles were obtained and analyzed after knocking down the gene transcripts by using siRNA technology. Global gene expression profiles were obtained from HS5 marrow stromal cells after knocking down mRNA of CDCP1, KDM3B or SDC1 by using siRNA technology.

2013-12-10 | E-GEOD-53199 | biostudies-arrayexpress

RNAi profiling of human MDA-MB-231 cells knocked down for RhoGDIbeta

Project description:RhoGDIbeta (ARHGDIB) is often expressed in tumor cells. It negatively regulates Rho-GTPases, but may have other functions as well. To analyze its effect on gene expression, RhoGDIbeta was suppressed by RNA interference in MDA-MB-231 breast cancer cells and changes in gene expression monitored by cDNA microarrays. Experiment Overall Design: MDA-MB-231 cells were either transfected with a RhoGDIbeta-specific siRNA or a control siRNA (siLuc), grown for three days, harvested and lysed for total RNA isolation. RNA was subjected to cDNA microarray analysis by using Affymetrix chip HG-U133A. A total of three independent transfection experiments were performed. The samples obtained from experiments 1, 2 and 3 were designated as siLuc_rep1, siLuc_rep2, siLuc_rep3 (siLuc transfected) and siARH_rep1, siARH_rep2, siARH_rep3 (siRhoGDIbeta transfected), respectively.

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siRNA-mediated HNRPA2B1 knock-down in MDA-MB-231 cells

Project description:Transcriptional profiling of MDA-MB-231 comparing the siRNA-mediated HNRPA2B1 knock-downs versus mock-transfected controls

2012-02-28 | GSE35757 | GEO