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Differential expression of E.coli mar/rob/soxS triple mutant and wild type in a mouse model of pyelonephritis

ABSTRACT: Mutation of marA, rob, and soxS causes a clinical strain of E.coli to be attenuated at d3 post-infection in a mouse model of pyelonephritis, here we extract RNA at d2 post infection to analyze transcriptional differences between the two strains.

ORGANISM(S): Escherichia coli

PROVIDER: GSE23417 | GEO | 2010/10/01

SECONDARY ACCESSION(S): PRJNA131115

REPOSITORIES: GEO

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Differential expression of E.coli mar/rob/soxS triple mutant and wild type in a mouse model of pyelonephritis

Project description:Mutation of marA, rob, and soxS causes a clinical strain of E.coli to be attenuated at d3 post-infection in a mouse model of pyelonephritis, here we extract RNA at d2 post infection to analyze transcriptional differences between the two strains. In order to purify enough bacterial RNA to perform microarray, we chose a time point (day 2 post-infection) just before the triple mutant begins to decrease in bacterial load. Mouse kidneys were extracted, homogonized, and samples were pooled in RNALater (Ambion) before extraction and analysis of the transcriptomes

2010-10-01 | E-GEOD-23417 | biostudies-arrayexpress

Cappable-seq analysis of the Salmonella Typhimurium SL1344 transcriptome with overexpression of MarA, SoxS, Rob or RamA

Project description:Cappable-seq was used to map transcription start sites globally in Salmonella Typhimurium SL1344. In addition to naturally occurring plasmids pSLT and pCol1B9, the cells carry derivatives of plasmid pAMNF, or pAMNM, that drive low level constitutive expression of epitope tagged MarA, SoxS, Rob or RamA derivatives.

2023-03-29 | E-MTAB-12628 | biostudies-arrayexpress

ChIP-seq analysis of MarA, SoxS, Rob and RamA biding across the Salmonella Typhimurium SL1344 genome

Project description:ChIP-seq data for Salmonella Typhimurium strain SL1344. The strain was grown at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies (for SoxS, Rob and RamA) or anti Myc (for MarA) antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

2023-03-29 | E-MTAB-12627 | biostudies-arrayexpress

Escherichia coli

Project description:Differential expression of E.coli mar/rob/soxS triple mutant and wild type in a mouse model of pyelonephritis

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Wild type E.coli SE15 vs. LuxS mutant E.coli SE15

Project description:Transcriptional profiling of E.coli SE15 comparing wild type E.coli SE15 with Autoindecur 2 synthesis gene LuxS mutnat E.coli SE15. E.coli SE15 is isolated from indwelling catheter of urinary tract infected patient. Examine change of quorum sensing related gene by deleting autoinducer 2 synthesis gene LuxS in E.coli

2013-08-02 | GSE49474 | GEO

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Wild type E.coli SE15 vs. LuxS mutant E.coli SE15

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Project description:Asta E.coli

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Comparison of IS5 Deletion mutant to E.coli Motile and NonMotile Isolates

Project description:These series of experiments compares the expression profile of the motility variants of E.coli MG1655 ( Motile and NonMotile isolates) to an isogenic E.coli MG1655 strain in which the IS5 upstream of flhDC has been deleted. The expression profiles of genes in the E.coli MG1655 motile isolate and E.coli MG1655 Non_Motile isolate also compared. Keywords: parallel sample

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