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Gene expression array of irradiated and/or lithium exposed iPSC-derived human neural progenitor cells

ABSTRACT: RNA isolation was performed using the RNeasy Mini Kit (Qiagen), per manufacturer instructions. For differential gene expression analysis, the Affymetrix Whole Transcript Assay with the Clariom S cartridge for human samples (n=2, BEA core facility, Sweden) was used. Cells were irradiated in an CIX2 Xstrahl X-Ray Irradiator with Aluminum filter at a focus-to-skin distance (FSD) of 40 cm and with an energy of 195kV, 10 mA, for and absorbed dose of 4 GY. To allow for assessment of irradiation damage, cells remained in the incubator for 1 hour. Pre-warmed DMEM/F-12 was mixed with the appropriate amount of LiCl powder (Sigma-Aldrich, Cat# L9650-100G), passed through a 0.22 μm filter, and supplemented with 1% Penicillin-Streptomycin, 1% N2, 0.1% B27, 0.1% EGF, and 0.1% FGF2 to produce 3mM Lithium Chloride (LiCl) hNSPC medium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE234353 | GEO | 2023/06/30

REPOSITORIES: GEO

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Gene expression array of irradiated and/or lithium exposed iPSC-derived human neural progenitor cells

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