Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain IV

ABSTRACT: We used bulk RNA sequencing to investigate the astrocytic signature isolated from dead-neuron-injected 8-month-old APOE4-cKO mice compared with APOE4-KI mice

ORGANISM(S): Mus musculus

PROVIDER: GSE234480 | GEO | 2023/07/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain I

Project description:We used bulk RNA sequencing to investigate the molecular signature of astrocytes affected by PU.1 inhibitor in APP/PS1:APOE4 KI mice.

2023-07-31 | GSE234385 | GEO

Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain V

Project description:We used bulk RNA sequencing to investigate the molecular signature of astrocytes affected by microglial APOE4 in P301S mice.

2023-07-31 | GSE234485 | GEO

Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain III

Project description:We used bulk RNA sequencing to investigate the astrocytic signature of astrocytes affected by Lgals3 injection in microglia.

2023-07-31 | GSE234416 | GEO

Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain II

Project description:We used bulk RNA sequencing to investigate the astrocytic signature of astrocytes affected the transfer of MGnD microglia

2023-07-31 | GSE234413 | GEO

Gene expression profile of either Dead neuron+/Fcrls+/CD11b+/Ly6C- or Dead neuron-/Fcrls+/CD11b+/Ly6C- microglia from the brain II

Project description:We used bulk RNA sequencing to investigate the molecular signature of phagocytic and non-phagocytic microglia affected by APOE isoform (APOE3-KI vs. APOE3-cKO vs. APOE4-KI vs. APOE4-cKO)

2023-07-31 | GSE234350 | GEO

Gene expression profile of either Dead neuron+/Fcrls+/CD11b+/Ly6C- or Dead neuron-/Fcrls+/CD11b+/Ly6C- microglia from the brain I

Project description:We used bulk RNA sequencing to investigate the molecular signature of phagocytic and non-phagocytic microglia affected by APOE isoform (APOE3-KI vs. APOE4-KI)

2023-07-31 | GSE234348 | GEO

Gene expression profile of Fcrls+/CD11b+/Ly6C- microglia from the brain I

Project description:We used bulk RNA sequencing to investigate the molecular signature of microglia affected by PU.1 inhibitor in APP/PS1:APOE4-KI mice.

2023-07-31 | GSE234383 | GEO

RNAseq of isolated astrocytes and microglia from APPPS1E4fl/fl mice following astroycte APOE removal

Project description:The e4 allele of the apolipoprotein E (APOE) gene is the strongest genetic risk factor for late-onset Alzheimer's disease and has been shown to increase amyloid pathology relative to the presence of the e2 and e3 alleles. In the brain, apoE is primarily produced by astrocytes and under pathological conditions also by microglia. The cell-type-specific role of apoE in amyloid pathology, especially after amyloid plaque deposition, has not been fully elucidated. We generated APPPS1-21/Aldh1l1-Cre/ERT2/apoE4flox/flox and APPPS1-21/apoE4flox/flox mice. At 3.8-months-of-age, during the phase of rapid plaque growth, we administered tamoxifen to reduce astrocytic APOE4 and assessed mice at 6-months-of-age. One day before tamoxifen treatment, mice were injected with methoxy-X04, a blood-brain-barrier permeant fluorescent marker that labeled the pre-existing fibrillar amyloid plaques. By using this strategy, we were able to characterize pre-existing plaques prior to the loss of astrocytic APOE4 and to also analyze newly-formed amyloid plaques after the loss of astrocytic APOE4. Interestingly, astrocytic APOE4 deletion strongly reduced pre-existing plaques. It also prevented new plaque formation and decreased glial reactivity. Importantly, the removal of astrocytic APOE4 resulted in enhanced microglial and astrocytic phagocytic ability, which may contribute to the reduction of the amyloid pathology.

2021-09-30 | GSE183050 | GEO

APOE4 cell-specific mechanisms underlying cerebrovascular disorder precede neuronal and synaptic dysfunction and cognitive deficits in mice

Project description:We performed single-nucleus RNA-sequencing of the cortex of APOE3 and APOE4 knock-in (KI)flox/flox mice to investigate the effects of human APOE4 gene on cell-specific signaling mechanisms at the BBB and in the brain.

2022-08-22 | GSE185063 | GEO

Human astrocyte secretome data

Project description:In late-onset sporadic Alzheimer’s disease (AD), the most prevalent and strongest risk factor is the ε4 allele of the apolipoprotein E (APOE4). To investigate the pathophysiological effects of the APOE4 genotype in the human cellular context, we generated isogenic iPSC-derived astrocytes bearing APOE4 alleles from APOE3 control iPSC. Next, to identify astrocytic APOE4-specific secreted proteomes, we performed a mass spectrometry using culture media from human astrocytes carrying APOE3 or APOE4.

2023-09-01 | PXD044308 | JPOST Repository

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data