Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential Gene Expression between YTN16 Gas6 Cells and YTN16 Cells

ABSTRACT: We have generated Gas6 knock-down YTN16 cells using the shRNA system to specifically target Growth arrest-specific 6 (Gas6), a gene of interest in mouse gastric cancer cells. Additionally, we aimed to determine the differences in gene expression levels between YTN16 cells and YTN16 cells with Gas6 knock-down (shGas6) through RNA-sequencing analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE234557 | GEO | 2023/06/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome of murine gastric cancer treated with anti-IL-17, PD-1 or CTLA-4 antibodies.

Project description:YTN2 and YTN16 cells are chemically induced gastric cancer cell lines. YTN2 is spontaneously regressed in C57BL/6 mice in CD8+ T cell-dependent manner, while YTN16 grows progressively. To investigate the interaction of tumor microenvironment and T cells, bulk RNA-Seq of total tumor RNA were performed in YTN2 and YTN16 gastric tumor-bearing mice.

2020-10-28 | GSE146027 | GEO

Targeted single cell RNA sequencing of T cells infiltrated in mouse gastric cancer.

Project description:YTN2 and YTN16 cells are chemically induced gastric cancer cell lines. YTN2 is spontaneously regressed in C57BL/6 mice in CD8+ T cell-dependent manner, while YTN16 grows progressively. To investigate tumor infiltrating T cells, we performed single-cell RNA-Seq of CD4+ and CD8+ T cells in YTN2 and YTN16 gastric tumor-bearing mice for 44 T cell-related genes (Supplementary Table 1).

2020-10-28 | GSE152888 | GEO

Inactivation of Gas6/Axl signaling aggravated Microglia-Astrocyte cross regulation after spinal cord injury

Project description:Aggressive inflammation and excessive scar formation are the main cause to the difficulty of neural tissue repair after spinal cord injury (SCI). Microglia and astrocytes act as important role in this micro-environment of SCI and there is cross regulation between microglia and astrocytes. After SCI, MG0 polarized into MG1 and MG3 which were belong to pro-inflammatory phenotype microglia; Naive astrocytes polarized into Reactive and Scar-forming astrocytes. Growth arrest-specific 6 (Gas6) and its receptor Axl was declined in all these cells after SCI. In vitro study, Gas6 had the negative effect on cytotoxic astrocytes and pro-inflammatory microglia polarization and even the cross regulation between cytotoxic astrocytes and pro-inflammatory microglia. Further mechanism study indicated that Gas6 suppressed cytotoxic phenotype polarization of astrocytes through inhibited the activation of YES-associated protein (YAP) signal pathway and suppressed pro-inflammatory phenotype polarization of microglia through inhibited the activation of NF-κB/p65 and JAK/Stat3 signal pathways. In vivo study, Gas6 treatment suppressed cytotoxic astrocytes and pro-inflammatory microglia polarization at the injured site of spinal cord to facilitate tissue repair and loco-motor recovery.

2023-10-21 | GSE245470 | GEO

Single-cell RNAseq of CD45+ cells in murine gastric cancer.

Project description:YTN2 and YTN16 were chemically induced gastric cancer cell lines. When inoculated subcutaneously, YTN2 spontaneously regresses while YTN16 grows progressively. By single-cell RNA-Seq and flow cytometry, we found more IL-17 producing cells in YTN16 than YTN2. Blockade of IL-17 signaling in YTN16 tumor-bearing mice suppressed tumor growth. To investigate tumor-infiltrating cells after IL-17 blockade, we performed single-cell RNA-Seq of CD45+ cells in YTN16 tumors.

2020-10-28 | GSE156725 | GEO

Gene expression in wild type and Gas6-/- endothelial cells co-cultured with cancer cells

Project description:Endothelial cells from 3 WT and 3 Gas6-/- mice were harvested, and were co-cultured with M27 murine lung cancer cells for 48 hours. Control experiments included untreated endothelial cells, and endothelial cells co-cultured with cancer cell media.

2016-08-02 | E-MTAB-3305 | biostudies-arrayexpress

Quantitative tyrosine phosphoproteome profiling of GAS6-AXL signaling network

Project description:Overexpression and amplification of AXL receptor tyrosine kinase (RTK) has been found in several hematologic and solid malignancies. Activation of AXL by its ligand GAS6 can enhance tumor-promoting processes such as proliferation, migration, invasion, and survival. Despite the important role of AXL in cancer development, a deep and quantitative mapping of its signaling transduction has not been performed. Here, we used a mass spectrometry-based quantitative proteomic approach to characterize the temporal dynamics of the phosphotyrosine proteome of MDA-MB-231 cells stimulated with GAS6

2021-08-24 | PXD025457 | Pride

Corticosterone inhibits Gas6 to govern hair follicle stem cell quiescence

Project description:Chronic, sustained exposure to stressors can profoundly impact tissue homeostasis, although the mechanisms by which these changes occur are largely unknown. Here, we report the adrenal gland-derived stress hormone corticosterone (the rodent equivalent of cortisol) regulates hair follicle stem cell (HFSC) quiescence and hair growth in mice. Without systemic corticosterone, HFSCs enter substantially more rounds of the regeneration cycle throughout life. Conversely, under chronic stress, elevated corticosterone levels prolong HFSC quiescence and keep hair follicles in an extended resting phase. Mechanistically, corticosterone acts on dermal papilla (DP) to suppress the expression of a secreted factor, Growth Arrest Specific 6 (Gas6). Restoring Gas6 expression overcomes stress-induced inhibition of HFSC activation and hair growth. Our work identifies corticosterone as a systemic inhibitor of HFSC activity via its impact on the niche, and demonstrates that removal of such inhibition drives HFSCs into frequent regeneration cycles with no observable defects long-term.

2021-02-13 | GSE135705 | GEO

Potential role of gas6 in zebrafish hindbrain development

Project description:identification of differentially expressed genes in gas6 homozygous mutant hindbrain when compared to wildtype hindbrain in zebrafish

2018-07-15 | GSE113396 | GEO

Effect of Gas6/Axl on HCC cell line gene expression

Project description:We adressed the impact of the receptor tyrosine kinase Axl and ist ligand Gas6 on gene expression in cellular HCC models, including cells harbouring Axl KO and exogenous reexpression.

2023-04-20 | GSE229731 | GEO

Expression profiles of murine gastric cancer cell lines

Project description:YTN2 and YTN16 cell lines are subclones established from chemically induced gastric cancer (Yamamoto M et al. Cancer Science 2018). When inoculated to normal C57BL/6 mice, YTN2 spontaneously regresses , while YTN16 grows progressively. To compare expression profiles of these cell lines, we examined microarray analysis.

2020-10-28 | GSE153231 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data