Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin remodeling events associated with gARID are essential for gametocyte development in Plasmodium [ChIP-seq]

ABSTRACT: To investigate the genome-wide binding sites of gARID in gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused gARID were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of gARID were determined from the sequence data.

ORGANISM(S): Plasmodium berghei ANKA

PROVIDER: GSE235409 | GEO | 2023/06/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

Project description:To investigate the genome-wide binding sites of the transcriptional regulators, PbAP2-FG2 and AP2R-2 in female gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-FG2 (PbAP2-FG2::GFP) or GFP-fused AP2R-2 (AP2R-2::GFP) were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data.

2023-02-02 | GSE213775 | GEO

Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [ChIP-seq]

Project description:To investigate the genome-wide binding sites of early zygote AP2-family transcription factor, AP2-Z in Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused AP2-Z (AP2-Z::GFP) were harvested at 6 hours post starting ookinete culture (hpoc) and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of AP2-Z were determined from the sequence data.

2022-08-05 | GSE198070 | GEO

Plasmodium berghei transcriptome for female gametocytes, male gametocytes, and asexual erythrocytic stages

Project description:Plasmodium berghei transcriptome for female gametocytes, male gametocytes, and asexual erythrocytic stages

2017-08-21 | GSE102167 | GEO

Chromatin remodeling events associated with gARID are essential for gametocyte development in Plasmodium [RNA-seq]

Project description:Plasmodium berghei transcriptomes of wild-type ANKA (WT) and garid knockout parasites [garid(-)] were analyzed at the gametocyte stage.

2023-06-30 | GSE235411 | GEO

Plasmodium berghei Gametocytogenesis

Project description:Transcriptional profiling of gametocyte non-producer lines in Plasmodium berghei Transcriptome of gametocyte non producer lines (natural and genetic KO) and parental (820) lines. The aim of the study was to identify key genes involved in the decision to commit to gametocytogenesis in Plasmodium berghei. These microarrays compare naturally selected lines that do not produce gametocytes, and the parental line and additionally a genetic knock out of AP2-G PBANKA_143750. Data published Sinha, Hughes, et, al Nature tbc.

2013-12-13 | GSE53246 | GEO

Plasmodium berghei Gametocytogenesis

2013-12-13 | E-GEOD-53246 | biostudies-arrayexpress

Homeodomain-like domain of Plasmodium berghei HDP1 binds to TGCACA motif.

Project description:To investigate the DNA-binding property of homeodomain-like domain of Plasmodium berghei HDP1, DNA immunoprecipitation followed by high-throughput sequencing (DIP-seq) analysis were performed. Recombinant homeodomain-like domain fused with glutathione S-transferase were mixed with the P. berghei genomic DNA fragmented via sonication, and protein-DNA complex was harvested using glutathione-sepharose resin. The obtained DNA fragments were sequenced via the next generation sequencing.

2024-02-25 | GSE256093 | GEO

Global expression profiling reveals shared and distinct transcript signatures in arrested act2(-) and CDPK4(-) Plasmodium berghei gametocytes

Project description:Gametocytogenesis and gametogenesis in malaria parasites are complex processes of cell differentiation and development likely involving many gene products. Gametocytes develop in the blood of the vertebrate host but mature gametocytes are not activated until taken up by the mosquito vector. Several distinct mutants have been described that block gametogenesis but the detailed molecular causes for the mutant phenotypes are not understood. To investigate whether a block in gametogenesis also results in a changed transcriptional profile, we studied two gene deletions mutants; act2(-) lacking stage-specific actin II and CDPK4(-) lacking calcium-dependent protein kinase 4. Whole-genome microarray analysis was performed from RNA of mature gametocytes to compare the transcriptomes of the mutants with wild-type Plasmodium berghei. The microarray analysis identified ~12% of all genes being differentially expressed in either or both mutants compared to normal gametocytes, as defined by at least two-fold change in transcript abundance. A large proportion of differentially expressed genes in both mutants overlapped consistent with the developmental gametocyte arrest. Distinct profiles in each mutant were also observed. Microarray experiments were performed as dual-color hybridizations on Agilent-024169 custom whole genome Plasmodium berghei 44K arrays. To compensate for dye-specific effects, a dye-reversal color-swap was applied.

2015-09-15 | E-GEOD-65032 | biostudies-arrayexpress

PFG controls gene expression in female gametocytes cooperatively with AP2-FG [AP2-FG_DIP]

Project description:AP2-FG is a female-specific transcription factor (TF) that plays an essential role in female gametocyte development. AP2-FG activates hundreds of genes by binding to a female-specific ten-base cis-acting element. Here, we report that in the rodent malaria parasite Plasmodium berghei, another female-specific TF designated as a partner of AP2-FG (PFG), controls gene expression in female gametocytes cooperatively with AP2-FG. Transcriptional mechanisms were analyzed in Plasmodium berghei female gametocytes.

2024-01-22 | GSE226020 | GEO

PFG controls gene expression in female gametocytes cooperatively with AP2-FG [PFG_ChIP-seq]

2024-01-22 | GSE226021 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data