Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-nucleus RNA sequencing revealed changes in microenvironment of epididymal adipose tissue during the development of obesity in mouse

ABSTRACT: Obesity causes the change of microenvironment of adipose tissue and induces the occurrence of adipose tissue inflammation. The objective of this study was to analyze the internal mechanism of microenvironment change in mouse parepididymal adipose tissue by single-nucleus RNA sequencing. Nutritional obesity mouse model was established and verified by morphology, blood index and liver oil red O staining. Select Ctrl group, Mid_Ob group and Ob group, and take 8 mice at 3 time points. The changes of cell population, gene and signaling pathway expression in the adipose tissue of the parastriatic muscle in mice during obesity were detected by single-nucleus RNA sequencing and immunohistochemistry. According to the results of single-nucleus RNA sequencing, adipose tissue was divided into fibroblasts, macrophages, neutrophils and endothelial cells. It is found that all cell types have changes during the development of obesity and play an important role in regulating the microenvironment of adipose tissue.

ORGANISM(S): Mus musculus

PROVIDER: GSE235469 | GEO | 2023/06/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Plasticity of epididymal adipose tissue in response diet-induced obesity at single-nucleus resolution.

Project description:Adipose tissues display a remarkable ability to adapt to the dietary status. Here, we have applied single-nucleus RNA-seq to map the plasticity of mouse epididymal white adipose tissue at single cell resolution in response to high-fat diet-induced obesity. The single-nucleus approach allowed us to recover all major cell types and to reveal distinct transcriptional stages along the entire adipogenic trajectory from preadipocyte commitment to mature adipocytes. We demonstrate the existence of different adipocyte subpopulations and show that obesity leads to disappearance of the lipogenic subpopulation and increased abundance of the stressed lipid scavenging subpopulation. Moreover, obesity is associated with major changes in the abundance and gene expression of other cell populations, including a dramatic increase in lipid handling genes in macrophages at the expense of macrophage-specific genes. The data provide a powerful resource for future hypothesis-driven investigations of the mechanisms of adipocyte differentiation and adipose tissue plasticity.

2021-01-05 | GSE160729 | GEO

Plasticity of epididymal adipose tissue in response diet-induced obesity at single-nucleus resolution.

Project description:Plasticity of epididymal adipose tissue in response diet-induced obesity at single-nucleus resolution.

| PRJNA674314 | ENA

Next generation sequencing analysis of epididymal adipose tissue (I)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity. We use Next generation sequencing analysis of mice fed with a control chow diet or a high fat diet.

2016-09-06 | E-GEOD-76133 | biostudies-arrayexpress

Next generation sequencing analysis of epididymal adipose tissue (II)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity. We use Next generation sequencing analysis of eWAT from control and Nova1 and Nova2-deficient mice fed with a control diet

2016-09-06 | E-GEOD-76294 | biostudies-arrayexpress

Next generation sequencing analysis of epididymal adipose tissue (III)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity. We use Next generation sequencing analysis of eWAT from control and Nova1 and Nova2-deficient mice fed with a high fat diet.

2016-09-06 | E-GEOD-76317 | biostudies-arrayexpress

Expression data from mice epididymal adipose tissue

Project description:Visceral white adipose tissue is closed correlated with obesity and metabolic dysfunction. Epididymal adipose tissue (eWAT) is considered as typical visceral white adipose tissue. Induction of browning of white adipose tissue improves metabolic dysfunction such as insulin resistance. In contrast to mice subcutaneous adipose tissue, visceral fat do not show significant browning under 4°C. However,under physiologically tolerable low temperature visceral adipose tissue can turn brown. We used microarrays to detail the global programme of gene expression in C57Bl/6 mice epididymal adipose tissue exposed to thermoneutral 30°C, 4°C and temperatures lower than 4°C.

2017-06-12 | GSE74062 | GEO

Robust single nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity

Project description:Single nucleus RNA sequencing (snRNA-seq), an alternative to single cell RNA sequencing (scRNA-seq), encounters technical challenges in obtaining high-quality nuclei and RNA, persistently hindering its applications. Here, we present a robust technique for isolating nuclei across various tissue types, remarkably enhancing snRNA-seq data quality. Employing this approach, we comprehensively characterize the depot-dependent cellular dynamics of various cell types underlying adipose tissue remodeling during obesity. By integrating nuclear RNA-seq data from adipocyte nuclei of varying sizes, we identify distinct adipocyte subpopulations categorized by size and functionality. Specifically, we characterize dysfunctional hypertrophic adipocytes prevalent in visceral adipose tissues during obesity, exhibiting cellular stress, inflammation and impaired metabolic gene expression. Obesity-induced changes in gene expression profiles of adipocyte subpopulations reveal their distinct contributions to adipose tissue pathophysiology. Our study establishes a robust snRNA-seq method, providing novel insights into the mechanisms orchestrating adipose tissue remodeling during obesity, with broader applicability across diverse biological systems.

2024-04-01 | GSE261417 | GEO

Epididymal Adipose Tissue Profiling In HMDP

Project description:Identify genes in the epididymal adipose tissue whose expression is under genetic regulation in the hybrid mouse diversity panel. The hybrid mouse diversity panel is comprised of classical inbred and recombinant inbred wild type mice. The RMA values of genes were used for genome wide association as described in Bennett et al Genome Research 2010. These data are used to identify candidate genes at loci associated with obesity and dietary responsiveness. GWAS for expression of epididymal adipose tissue in inbred strains

2013-01-09 | E-GEOD-42890 | biostudies-arrayexpress

Next generation sequencing analysis of epididymal adipose tissue (III)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity.

2016-09-06 | GSE76317 | GEO

Next generation sequencing analysis of epididymal adipose tissue (II)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity.

2016-09-06 | GSE76294 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data