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Effect of deficiency of Elk4 on transcriptome changes in murine bone marrow derived mast cells upon FcϵRI mediated activation

ABSTRACT: The proliferative potential of mast cells after activation for 3-4h was found to be decreased, which suggests that mast cell degranulation and cell proliferation are differentially regulated. ELK4, a member of the ternary complex factor (TCF) subfamily of Ets transcription factors, is one of the downstream effectors of MAPK signaling that is critical for cell proliferation. And Elk4 has been identified to be vital for macrophage activation in response to zymosan and the transcriptional response to 12-O-tetrade canoyl phorbol-13-acetate (TPA) stimulation in fibroblast. However, the effect of ELK4 on the mast cell transcriptional response to FcϵRI and GPCR mediated activation and its potential functional significance in mast cells remain unclear. Here, We characterised the transcriptional program downstream of FcϵRI signalling using IgE-DNP/HSA stimulation by RNA-seq. The investigation of the transcriptional response was performed using murine bone marrow derived mast cells(BMMCs) from different animals: (i) wild type mice, (ii) Elk4 heterozygous mice, (iii) Elk4 knockout mice. Overall, our study identifies a new physiological role of the transcription factor ELK4 in mast cell proliferation and activation.

ORGANISM(S): Mus musculus

PROVIDER: GSE236014 | GEO | 2023/07/12

REPOSITORIES: GEO

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Homo sapiens

Project description:Mast Cell Activation

| PRJNA92929 | ENA

Project description:anaphylaxis and mast cell activation after IgE and Ag stimulation

| PRJNA678624 | ENA