Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of loperamide on transcriptional profile of Raw264.7 cells infected with Salmonella

ABSTRACT: Our previous screening allowed us to identify the loperamide (LPD) as a potent inhibitor of S. Typhimurium intracellular proliferation. LPD treatment of infected cells markedly promoted the host autophagic response and lysosomal activity. A mechanistic study revealed that the elevated host autophagy and elimination of intracellular bacteria were dependent on the high expression of glycoprotein nonmetastatic melanoma protein B (GPNMB) induced by LPD. In addition, LPD treatment could effectively protect S. Typhimurium-infected LPD Galleria mellonella and mouse models. Thus, our study suggests that LPD may be used for the treatment of diseases caused by intracellular bacterial pathogens. Moreover, LPD may serve as a promising lead compound for the development of anti-infection drugs based on the HDT strategy.

ORGANISM(S): Mus musculus

PROVIDER: GSE236340 | GEO | 2025/07/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

microRNA response of Galleria mellonella to Listeria monocytogenes infection: a powerful method to predict protein function in mammals exemplified by the autophagy adaptor optineurin

Project description:The Galleria mellonella larvae were infected with Listeria monocytogenes and on the 5th of post infection RNA is isolated from infected and non-infected control larvae. RNA samples were processed for miRNA profile in response to L. monocytogenes infection in Galleria mellonella larvae.

2017-10-30 | E-MTAB-4024 | biostudies-arrayexpress

Simultaneous Detection of Gene Expression of insects, nematode and bacterial specific genes to monitor the infection.

Project description:In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. Variations in gene expression were seen as per the expectation.

2024-08-31 | GSE197737 | GEO

MicroRNAs regulate differential innate immune response to uropathogenic and commensal-like Escherichia coli infection in the surrogate insect model Galleria mellonella

Project description:Identification and expression analysis of microRNAs in infected larvae of the insect model Galleria mellonella with uropathogenic (UPEC) and commensal E. coli strains that are known to cause symptomatic and asymptomatic bacteriuria (ABU) in humans, respectively.

2018-12-18 | GSE123965 | GEO

Triptolide clears Staphylococcus aureus infection by targeting XIAP to induce host apoptosis while preserving gut microbiota homeostasis [mouse lung macrophages]

Project description:Staphylococcus aureus (SA) remains a global health threat due to its increasing drug resistance and intracellular persistence, which compromise the efficacy of conventional antibiotics. Host-directed therapy (HDT) has emerged as a promising alternative by modulating host immunity. With the multi-targeting and immunomodulatory properties, Traditional Chinese Medicine (TCM) monomers represent ideal candidates for HDT. However, their ability to promote host immunity-mediated SA clearance remains largely unexplored. Here, we identified triptolide as a compound that facilitates intracellular clearance of SA and MRSA. Mechanistic studies revealed that triptolide directly binds to the X‑linked inhibitor of apoptosis protein (XIAP) to inhibit XIAP-caspase interaction, thereby releasing caspase inhibition and inducing apoptosis. Moreover, treatment with triptolide reduced bacterial loads and alleviated lung inflammation in mice infected with SA and MRSA, concurrently maintaining microbiota homeostasis and improving metabolic function. Taken together, these findings establish triptolide as a promising lead compound for the development of HDT against SA and MRSA infections.

2026-05-18 | GSE320244 | GEO

Triptolide clears Staphylococcus aureus infection by targeting XIAP to induce host apoptosis while preserving gut microbiota homeostasis [iBMDMs]

2026-05-18 | GSE320243 | GEO

Project description:Galleria mellonella microbiome

| PRJNA730794 | ENA

Galleria mellonella

Project description:Galleria mellonella overview

| PRJNA246333 | ENA

Galleria mellonella isolate:WM207_F2

Project description:Galleria mellonella genome

| PRJNA893711 | ENA

Galleria mellonella