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Small RNA-seq gene expression profile of the SUM159PT breast cancer cell line, upon overexpression of the microRNA responsive elements of candidate TDMD triggers (ABCA1, HADHB, TRAF6)

ABSTRACT: We investigate the potential of candidate microRNA responsive elements (MRE, contained in the 3'UTR of candidate transcripts) to trigger target-dependent microRNA degradation (TDMD). To this purpose, we over-expressed the MRE by delivering a lentiviral construct in SUM159PT cells and we profiled microRNAs by small RNA sequencing 7 days after the infection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE236781 | GEO | 2026/06/30

REPOSITORIES: GEO

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small RNA-seq gene expression profile of the SUM159PT breast cancer cell line, upon overexpression of the microRNA responsive elements of candidate TDMD triggers (ABCA1, HADHB, TRAF6)

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Endogenous transcripts control miRNA levels and activity in mammalian cells by a target-dependent miRNA degradation mechanism [RNA-Seq]

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Endogenous transcripts control miRNA levels and activity in mammalian cells by a target-dependent miRNA degradation mechanism [miRNA-Seq]

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Endogenous transcripts control miRNA levels and activity in mammalian cells by a target-dependent miRNA degradation mechanism [RNA-Seq_2]

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Ribosome profiling on SUM159PT cells grown in vitro or in vivo

Project description:SUM159PT cells were grown either in vitro (in culture) or in vivo (mouse), after which RPL10a tagged with GFP was used to perform extraction by immoprecipitation and subsequent ribosome profiling

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RNA-seq gene expression profile of SUM159PT breast cancer cell line upon manipulation of two TDMD triggers, NREP and SERPINE1

Project description:We investigate the effect of the perturbation of two transcripts involved in target-dependent microRNA degradation (TDMD): NREP is a trigger for miR-29b; SERPINE1 is a trigger for miR-30. We perturbed the genes and monitored the activity of miR-29 or miR-30 analysing the expression changes of their predicted target genes by RNA sequencing.

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Gene expression profile at single cell level of SUM149PT, SUM185PE, SUM229PE, SUM159PT and SUM1315MO2 cell lines

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Gene expression profiling of MDA231, BT549, and SUM159PT cells after selumetinib treatment or DUSP4 siRNA knockdown

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