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Micro-patterned culture of iPSC-derived alveolar epithelial cells [alveolar]

ABSTRACT: We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, the iPSC-derived lung progenitor cells were differentiated into alveolar epithelial cells in a position-specific manner.

ORGANISM(S): Homo sapiens

PROVIDER: GSE236839 | GEO | 2024/02/29

REPOSITORIES: GEO

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Micro-patterned culture of iPSC-derived airway epithelial cells [airway]

Project description:We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, the iPSC-derived lung progenitor cells were differentiated into airway epithelial cells in a position-specific manner.

2024-02-29 | GSE236840 | GEO

The iPSC-derived pulmonary epithilial cells in micro-patterned culture plate

Project description:We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, various types of lung epithelial cells were identified.

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Human iPSC-derived pulmonary epithelial cells infected with SARS-CoV-2 variants in the micro-patterned culture plates [SARS-CoV-2]

Project description:We infected iPSC-derived pulmonary epithelial cells in the micro-patterned culture plate with the SARS-CoV-2 variants. Analyses of infected alveolar and airway epithelial cells, respectively, revealed the tropism of the variants.

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Phenotype modulation of VSMCs on patterned substrate

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Kidney micro-organoids generated in suspension culture

Project description:Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scale-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Each micro-organoid contains 6-10 nephrons surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. Ligand-receptor mapping identified TGFβ signalling between stromal and epithelial cell types as likely to result in fibrotic changes observed with long-term culture. The addition of an ALK4 inhibitor suppressed stromal expansion, maintaining epithelial morphology and improving maturation. This suspension culture micro-organoid methodology resulted in a 3-4-fold increase in final cell yield compared to static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.

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Adult Leptomeninges are Regionally Patterned and Provide Specialized Support to Underlying Brain Tissue

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Project description:This SuperSeries is composed of the SubSeries listed below.

2024-02-29 | GSE236842 | GEO

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