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Next generation sequencing facilitates quantitative analysis of transcriptional profiling of clinical strain Candida albicans CA10 with iravacycline hydrochloride and fluconazole treament alone or in combination.

ABSTRACT: mRNA profiles of Candida albicans clinical strain CA10, which treated with iravacycline hydrochloride and fluconazole alone or in combination, were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000 . The sequence reads that passed quality filters were analyzed at the transcript isoform level. qRT–PCR validation was performed using SYBR Green assay.We found that the gene expression related to purine nucleotide synthesis was significantly down-regulated in the combination group of iravacycline hydrochloride and fluconazole, which suggested that we might have DNA damage.

ORGANISM(S): Candida albicans

PROVIDER: GSE237112 | GEO | 2026/07/12

REPOSITORIES: GEO

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