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Regulation of mRNA translation by RNH1 in hematopoietic cells

ABSTRACT: Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) containing protein. It binds to pancreatic-type ribonucleases and inhibit their function. However, the entire biological role of Rnh1 is unknown. We generated RNH1 knock out K562 cells by CRISPR/Cas9 method. We isolated polysomal RNA from control and RNH1-deficient K562 cells to quantify actively translated mRNAs by RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE237169 | GEO | 2024/03/20

REPOSITORIES: GEO

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