Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression by RNA-seq in purified paneth cells of WT and panetch cell specific P55 KO mice 3 hours after TNF

ABSTRACT: The study of how gene expression in paneth cells changes 3 hours after TNF admistration in mice were the TNFR1 (P55) genes was deleted in paneth cells using Defa6-cre.

ORGANISM(S): Mus musculus

PROVIDER: GSE237759 | GEO | 2023/12/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

transcriptome of purified PC and non-PC fractions: GR-WT & GR-KO

2024-06-17 | GSE269510 | GEO

Gene expression by RNA-seq in purified paneth cells of WT and panetch cell specific P55 KO mice 3 hours after TNF

Project description:Gene expression by RNA-seq in purified paneth cells of WT and panetch cell specific P55 KO mice 3 hours after TNF

| PRJNA996563 | ENA

Gene expression by RNA-seq in Liver of WT and panetch cell specific P55 KO mice 18 hours after TNF

Project description:After a challenge with TNF, gene expression changes in the liver may result from direct effect of TNF, through liver TNF receptors, and indirect effect. Baterial infiltration of the liver due to leaky gut after TNF is a primary contributor of this effect. In order to evaluate only the direct effects of TNF on lier transcriptome, we performed TNF challenge in normal WT mice and P55 panetch cell KO mice, which do not show the leaky gut syndrome.

2023-12-15 | GSE237949 | GEO

PanethcellTNF-signalinginducesbacterialsepsis: PC transcriptome 15h ater TNF

Project description:Paneth Cells (PCs) are secretory cells located in the crypts of Lieberkühn of the small intestine. They produce antimicrobial peptides (AMPs) and are thought to keep the microbiome under control. The cytokine TNF, which functions mainly via its TNF receptor 1 (TNFR1 or P55), is a driving force in many inflammatory bowel disease (IBD) patients, but its impact on PCs is poorly known. We have generated PC-specific P55 knockout mice (P55Paneth KO) and found that these mice are protected against lethal TNF-induced systemic inflammatory response syndrome (SIRS). The impact of TNF on PCs is characterized by morphological changes and by intestinal bacterial translocation into organs, such as liver, in which these bacteria induce expression of numerous genes, associated with typical sepsis signatures. The presence of these bacteria cause lethality of the mice. Based on bulk RNA-SEQ on pure PCs, P55 signaling stimulated mainly a type-I IFN response in the PCs, which drives the bacterial translocation and lethality via IFNAR1. Interestingly, based on ER stress reporter mice, PCs constitutively have high amounts of spliced XBP1 (XBP1s) protein, presumably to support their secretion of AMPs, and TNF eradicates this XBP1s production, leading to failure of the unfolded protein response (UPR). This causes reduced antimicrobial activity in PCs, in a P55 and IFNAR1-dependent way. Our data suggest a novel axis induced by TNF in PCs, leading to interferon-induced UPR failure causing lethal bacterial translocation to the liver (and other organs)

2024-06-03 | GSE267790 | GEO

PanethcellTNF-signalinginducesbacterialsepsis: PC transcriptome 15h ater TNF in IFNARKO mice

2024-06-03 | GSE267927 | GEO

PanethcellTNF-signalinginducesbacterialsepsis: transcriptome of intestinal epithelial cells 8h after TNF

2024-06-05 | GSE268933 | GEO

Effect of TNF of gene expression in mouse paneth cells after 3 hours

Project description:The study of how gene expression in paneth cells changes 3 hours after TNF admistration. We used deep paired-end sequencing to also be able to investigate alternative splicing and non-coding RNA

2025-02-26 | GSE281683 | GEO

Shotgun proteomics on murine paneth cells after TNF-alpha treatment

Project description:Mouse were injected with TNF-alpha prior isolation of paneth cells with flow cytometry.

2024-07-18 | PXD053044 | Pride

Expression profiling of purified cells after (mature) paneth cell depletion.

Project description:We used transgenic mice expressing diphtheria toxin in a paneth cell specific manner to eablate paneth cells, but found that we could still detect and also purify (paneth) cells in the transgenic mice. We purified this population and a control WT population of paneth cells for expression profiling, with the goal to check if the diphtheria toxin expression system had low penetrance or if our PC population in TG mice was immature , and thus did not yet express the active diphtheria toxin.

2025-02-26 | GSE281800 | GEO

Paneth cell trancriptome: effect of DEX on TNF induced inflammatory response

Project description:We aimed to investiagte the protective anti-inflammatory effect of glucocorticoids (dexamethsone, DEX) on paneth cells from mice treated with TNF. TNF treatment causes leaky gut syndrome and paneth cell disfunction. Using pre-treatment with DEX we aim to protect paneth cell against TNF effects

2025-02-26 | GSE281876 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data