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Genes modulated by rhBMP2 and rhBMP7 during osteoblastic differentiation of C2C12 cells

ABSTRACT: This study analyses the gene expression profile of pre-osteoblastic C2C12 mice cells during osteodifferentiation in control and induced by rhBMP2 and rhBMP7 using DNA microarrays. Genes which were already known to be activated by BMP2, to be also activated by rhBMP7, indicating that BMP7 induces these genes in a similar fashion as BMP2. Moreover, we identified some new genes, such as Hoxc8, Glis1, Glis3, Ecsit, LrP6, Dvl1, Grn, BMP1, Ltbp2, Plod1, Htra1 and IGFBP-rP10, to be upregulated by both rhBMPs.

ORGANISM(S): Mus musculus

PROVIDER: GSE23783 | GEO | 2011/11/17

SECONDARY ACCESSION(S): PRJNA130723

REPOSITORIES: GEO

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Genes modulated by rhBMP2 and rhBMP7 during osteoblastic differentiation of C2C12 cells

Project description:This study analyses the gene expression profile of pre-osteoblastic C2C12 mice cells during osteodifferentiation in control and induced by rhBMP2 and rhBMP7 using DNA microarrays. Genes which were already known to be activated by BMP2, to be also activated by rhBMP7, indicating that BMP7 induces these genes in a similar fashion as BMP2. Moreover, we identified some new genes, such as Hoxc8, Glis1, Glis3, Ecsit, LrP6, Dvl1, Grn, BMP1, Ltbp2, Plod1, Htra1 and IGFBP-rP10, to be upregulated by both rhBMPs. Total RNA from mouse pre-myoblastic and pre-osteoblastic C2C12 cell line, after 12h of treatment with rhBMP2 and rhBMP7, was hybridized to a microarray and analyzed.

2011-11-17 | E-GEOD-23783 | biostudies-arrayexpress

Characterization of normal fibroblast transcriptomes upon transient silencing of LTBP2 by RNAseq

Project description:Purpose: Our single-cell RNAseq data show that LTBP2 is a marker of a cancer associated fibroblast subpopulation in colorectal cancer liver metastases. Moreover, MTT assays of cultured fibroblasts treated with anti-LTBP2 antibodies suggest that LTBP2 is essential for their survival. The goal of this study is to understand the role of LTBP2 in fibroblasts. Methods: Normal olon fibroblasts (CCD18Co) were transfected 48h with siRNA containing an anti-LTBP2 or a non target sequence. Cell monolayer total RNA of 3 biological replicates was then extracted and submitted to Illumina RNAseq using NovaSeq6000. Results: Transcriptomics revealed massive changes in gene expression when LTBP2 is knocked down: 496 genes were differentially expressed between the colon fibroblasts treated with siRNA anti-LTBP2 and NT. Those genes are implicated in diverse biological processes such as extracellular matrix organization, angiogenesis or cell adhesion. Conclusions: Our study explored LTBP2 functions in normal fibroblasts. RNAseq data support its important role. After 48h, LTBP2 silencing induces changes in gene expression of numerous genes involved in fibroblast functions.

2022-11-22 | GSE191323 | GEO

RNAseq experiment to identify differentially expressed genes in dvl/rtfl mutants and DVL overexpresion lines in inflorescence apices of Arabidopsis thaliana

Project description:The lack of visible phenotypes of multiple dvl/rtfl mutants prompted us to compare the transcriptomic signatures of inflorescence apices of wildtype and mutant combinations (specifically dvl1 dvl8 dvl11 rtfl11 dvl19 and dvl8 dvl11 rtfl9 rtfl11 dvl19), as well as dvl1-D mutant, where DVL1 is overexpressed, and 35Spro:DVL8 line. Significant changes in gene expression were caused by the mutation of DVL genes in both quintuple mutant and overexpression backgrounds.

2023-09-19 | GSE243205 | GEO

iPS cell generation

Project description:This SuperSeries is composed of the following subset Series: GSE26428: Effect of Glis1 on human iPS cell generation GSE26429: Promotion of Direct Reprogramming by Glis1 GSE26430: Effect of Glis1, Dmrtb1, and Pitx2 on mouse iPS cell generation Refer to individual Series

2011-06-10 | E-GEOD-26431 | biostudies-arrayexpress

Expression analysis of human adipose tissue stem cells (ADSCs) transduced with homeobox C8 (Hoxc8) or control vector, followed by treatment with adipogenic-induction media for 14 days

Project description:Adipogenesis occurs through a specific gene program in undifferentiated fat progenitors. We hypothesized that the properties of the fat progenitors are regulated by hox genes, the developmental genes essential in different tissue stem cells. Their biased expression in white and brown fat implies roles in distinguishing the two fat types. Among 39 Hox genes, Hoxc8 is highly enriched in undifferentiated adipose tissue stem cells (ADSCs) and down-regulated in differentiated adipocytes. Forced expression of Hoxc8 suppressed adipocyte differentiation of ADSCs. Using microarrays, we investigated the effect of Hoxc8 overexpression on global transcripts in ADSCs. We compared among four groups: untreated ADSCs, adipogenic induction media (MDI)-treated ADSCs, MDI-treated ADSC-vector and MDI-treated ADSC-Hoxc8. A number of, but not all, adipogenesis-related genes are suppressed by Hoxc8. This dataset illustrates the global effect of Hoxc8, a developmental transcription factor, on the expression of adipogenesis-related genes.

2010-06-18 | GSE22429 | GEO

Promotion of Direct Reprogramming by Glis1

Project description:Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of three transcription factors Oct3/4, Sox2, and Klf4 (OSK), albeit at a low efficiency. The protooncogene c-Myc enhances the efficiency of iPSC generation by OSK, but it also increases the tumorigenicity of the resulting iPSC. In the current study, we found the Gli-like transcription factor Glis1, when expressed together with OSK, to markedly enhance the generation of iPSC from both mouse and human fibroblasts. Mouse iPSC generated by OSK and Glis1 can form germline-competent chimeras. Glis1 is enriched in unfertilized oocytes and one cell-stage embryos. DNA microarray analyses revealed that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, mesenchymal-epithelial transition (MET), and Esrrb. These results therefore demonstrated that oocyte transcription factor Glis1 effectively promote direct reprogramming during iPSC generation. p53-null mouse embryonic fibroblasts were transduced with OSK and OSK+Glis1 and were used for microarray analyses.

2011-06-10 | E-GEOD-26429 | biostudies-arrayexpress

Effect of Glis1 on human iPS cell generation

Project description:Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of three transcription factors Oct3/4, Sox2, and Klf4 (OSK), albeit at a low efficiency. The protooncogene c-Myc enhances the efficiency of iPSC generation by OSK, but it also increases the tumorigenicity of the resulting iPSC. In the current study, we found the Gli-like transcription factor Glis1, when expressed together with OSK, to markedly enhance the generation of iPSC from both mouse and human fibroblasts. Mouse iPSC generated by OSK and Glis1 can form germline-competent chimeras. Glis1 is enriched in unfertilized oocytes and one cell-stage embryos. DNA microarray analyses revealed that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, mesenchymal-epithelial transition (MET), and Esrrb. These results therefore demonstrated that oocyte transcription factor Glis1 effectively promote direct reprogramming during iPSC generation. Adult human fibroblasts were transduced with OSKM and OSK+Glis1 and were used for microarray analyses.

2011-06-10 | E-GEOD-26428 | biostudies-arrayexpress

Effect of Glis1, Dmrtb1, and Pitx2 on mouse iPS cell generation

Project description:Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of three transcription factors Oct3/4, Sox2, and Klf4 (OSK), albeit at a low efficiency. The protooncogene c-Myc enhances the efficiency of iPSC generation by OSK, but it also increases the tumorigenicity of the resulting iPSC. In the current study, we found the Gli-like transcription factor Glis1, when expressed together with OSK, to markedly enhance the generation of iPSC from both mouse and human fibroblasts. Mouse iPSC generated by OSK and Glis1 can form germline-competent chimeras. Glis1 is enriched in unfertilized oocytes and one cell-stage embryos. DNA microarray analyses revealed that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, mesenchymal-epithelial transition (MET), and Esrrb. These results therefore demonstrated that oocyte transcription factor Glis1 effectively promote direct reprogramming during iPSC generation. Mouse embryonic fibroblasts were transduced with OSKM, OSM+Glis1, OSM+Dmrtb1, and OSM+Pitx2 and were used for microarray analyses.

2011-06-10 | E-GEOD-26430 | biostudies-arrayexpress

Glis1 Activate Glycolytic Process and Increase H3K27Ac Level on Pluripotent and Second Wave Genes Promoter Facilitating Pluripotency Induction [RNA-Seq]

Project description:Metabolic activity possess a crucial role during cell fate determination. Here, we show that maternal transcriptional factor Glis1 could not only promote normal MEF reprogramming but also enable aging MEF reprogramming. By association ChIP-seq and RNA-seq, we found that Glis1 could bind and activate glycolytic genes, and bind and suppress somatic state genes simultaneously during reprogramming. While knockdown phosphoglyceric kinase (Pgk1) impeded reprogramming efficiency which indicates that Glis1-glycolytic process axes is required for Glis1 function. Metabolic analysis indicated that the level of acetyl-CoA has a significant fluctuation upon Glis1 induction or silence. Acetate, a precursor of acetyl-CoA rescued the effects on reprogramming in a time-dependent manner when knockdown endogenous Glis1. The fluctuation of Ac-CoA changed the level of H3K27Ac, especially in pluripotent genes and “second wave” genes promoter, and activated the expression of endogenous pluripotency genes in advance subsequently. In conclusion, we demonstrate the exactly novel mechanism of Glis1 in pluripotency acquisition.

2020-06-24 | GSE131491 | GEO

Glis1 Activate Glycolytic Process and Increase H3K27Ac Level on Pluripotent and Second Wave Genes Promoter Facilitating Pluripotency Induction [ChIP-Seq]

2020-06-24 | GSE131426 | GEO

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