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Single-cell 5' RNA sequencing of Bactrian camel PBMCs

ABSTRACT: Camelids are capable of producing both conventional tetrameric antibodies (Abs) and dimeric heavy-chain antibodies (HCAbs). While B cells generating these two types of Abs exhibit distinct B-cell receptors (BCRs), it remains unclear whether these two B cell populations differ in their phenotypes and developmental processes. Here, we collected eight PBMC samples before and after immunization from four Bactrian camels and conducted single-cell 5’ RNA sequencing. We characterized the functional subtypes and differentiation trajectories of circulating B cells in camels, including native B cells, memory B cells, intermediate B cells, atypical B cells, and plasma cells. Additionally, we reconstructed single-cell BCR sequences and revealed the IGHV and IGHC gene types. We found that B cells with variable genes of HACbs (VHH) were widely present in various functional subtypes and showed highly overlapping differentiation trajectories to B cells with variable genes of conventional Abs (VH). After immunization, the transcriptional changes in VHH+ and VH+ B cells were also largely consistent. Our study not only elucidates the cellular context of HCAb production in camels, but also lays the foundation for the development of single B cell-based nanobody screening.

ORGANISM(S): Camelus bactrianus

PROVIDER: GSE238082 | GEO | 2024/04/15

REPOSITORIES: GEO

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Fc receptor-like 6 (FCRL6) defines pre-BCR dependent and independent pathways of natural antibody selection

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2020-02-26 | GSE132438 | GEO

Germline-Encoded Affinity for Cognate Antigen Enables Vaccine Amplification of a Human Broadly Neutralizing Response Against Influenza Virus

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Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice

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2020-05-26 | PXD015168 | Pride

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