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RNA sequencing analysis of IP (with GFP antibody) and Input samples of the mPFC of stressed DBA mice expressing GFP-L10a

ABSTRACT: We performed high-throughput profiling of gene expression in aPVT-projectors in the mPFC of DBA2/J mice subjected to subchronic and mild social defeat stress. DBA mice were subjected to 5-day of social defeat stress and then tested their social interaction and anhedonic state. After behavioral tests, mice were divided into five subtypes: SA, social interaction deficit, ANH: anhedonia, SA:ANH, both social dificit and anhedonia, RES, resilience and non-stressed (NS) control. Mice were euthanized for tissue collection. We then conducted the Immunoprecipitation and RNA-seq. Our study provided insights into the understanding of the molecular mechanisms underlying behavioral heterogeneity.

ORGANISM(S): Mus musculus

PROVIDER: GSE238142 | GEO | 2024/01/15

REPOSITORIES: GEO

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RNA sequencing analysis of gene expression in the mPFC of stressed DBA mice given vehicle of KDM5C inhibitor

Project description:We performed high-throughput profiling of gene expression in the mPFC of stress-vulnerable DBA2/J mice subjected to subchronic and mild social defeat stress.DBA mice were subjected to 5-day of social defeat stress and then injected with KDM5C inhibitor (KDM5Ci) or vehicle. After behavioral tests, mice were euthanized for tissue collection. We then conducted the transcriptomics analysis. Our study provided insights into the understanding of the molecular mechanisms underlying stress susceptibility and provide the role of KDM5C in stress-induced behavioral changes.

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GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency

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BAZ1B in Nucleus Accumbens Regulates Reward-Related Behaviors in Response to Distinct Emotional Stimuli

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2016-07-30 | E-GEOD-85005 | biostudies-arrayexpress

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