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Array CGH of breast cancer cells with distinct metastatic properties

ABSTRACT: Xenograft metastases from MDA-MB-231 cells and CN34 cells at different organs were collected and yielded sub-populations that exhibited increased metastagenicity to the lung or brain. Genomic aberrations of these subpopulations were profiled and compared to the parental populations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE23823 | GEO | 2010/12/12

SECONDARY ACCESSION(S): PRJNA130573

REPOSITORIES: GEO

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Array CGH of breast cancer cells with distinct metastatic properties

Project description:Xenograft metastases from MDA-MB-231 cells and CN34 cells at different organs were collected and yielded sub-populations that exhibited increased metastagenicity to the lung or brain. Genomic aberrations of these subpopulations were profiled and compared to the parental populations. Array CGH of breast cancer cells with distinct metastatic properties

2010-12-12 | E-GEOD-23823 | biostudies-arrayexpress

Breast cancer hybrid cells after spontaneous cell fusion with MSC display tumor dorming-like properties

Project description:Functional changes were investigated in vitro and in vivo following spontaneous fusion and hybrid cell formation in co-cultures of primary human mesenchymal stroma/stem-like cells (MSC) with human MDA-MB-231 breast cancer cells. Lentiviral fluorescence-labeled MSC with eGFP and breast cancer cells labeled with mcherry resulted in dual-fluorescing hybrid cells. Double FACS sorting and single cell cloning revealed different aneuploid hybrid populations (MDA-hyb1 MDA-hyb3, MDA-hyb5)

2020-12-31 | GSE157199 | GEO

Bone invigorates metastatic seeding [RNA-seq]

Project description:We herein examine the transciptioal alteration of a single cell derived MDA-MB-231 subline,SCP21. By retrieving tumor cells inoculated in different sites, we generated multiple mammary fat pad tumor, lung metastases or bone metastasis derived SCP21 sublines. RNA-sequencing uncovered the decreased expression of EZH2 target genes in bone metastases derived cells compared other organ entrained and parental SCP21 cells , suggesting enhanced EZH2 activity. Moreover, such phenotype is reversible upon in vitro culture. As a positive control, bone derived cells were also treated with EZH2 inhibitor, EPZ011989, to confirm the decreased level of EZH2 target genes upon EZH2 inhibition.

2021-04-29 | GSE160773 | GEO

Increased metastatic capacity of breast cancer cells after interaction and hybrid formation with mesenchymal stroma/stem cells (MSC)

Project description:Functional changes were investigated in vitro and in vivo following spontaneous fusion and hybrid cell formation in co-cultures of primary human mesenchymal stroma/stem-like cells (MSC) with human MDA-MB-231 breast cancer cells. Lentiviral fluorescence-labeled MSC with eGFP and breast cancer cells labeled with mcherry resulted in dual-fluorescing hybrid cells. Double FACS sorting and single cell cloning revealed two different aneuploid male hybrid populations (MDA-hyb1 and MDA-hyb2)

2018-02-13 | GSE100551 | GEO

The murine metastatic microenvironment of experimental brain metastases in breast cancer differs by host age in vivo: A proteomic study.

Project description:Breast cancer in young patients is known to exhibit more aggressive biological behavior and is associated with a less favorable prognosis than the same disease in older patients, owing in part to an increased incidence of brain metastases, the mechanistic explanations behind which remain poorly understood. We recently reported that young mice, compared to older mice, showed about a three-fold increase in the development of brain metastases in mouse models of triple-negative and luminal B breast cancer. Here we have performed a quantitative mass spectrometry-based proteomic analysis to identify proteins contributing to age-related disparities in the development of breast cancer brain metastases. Using a mouse model of brain-tropic (MDA-MB-231BR) triple-negative breast cancer, we harvested subpopulations of tumor metastases, the tumor-adjacent metastatic microenvironment, and uninvolved brain tissues via laser microdissection followed by quantitative proteomic analysis using high resolution mass spectrometry to characterize differentially abundant proteins contributing to age-dependent rates of brain metastasis.

2024-01-11 | PXD042346 | Pride

Micro-RNAs expressed in oligometastases inhibit genes involved with adhesion, invasion and motility

Project description:Analysis of MDA-MB-231 cells 48 hours after transfection with non-targeting control, micro-RNA mimics or corresponding micro-RNA inhibitor. The hypothesis tested was that micro-RNAs previously identified as expressed in oligometastatic patients' metastases at a higher level than polymetastatic patients' metastases suppress genes regulating adhesion, motility, invasion and growth. Results showed that multiple micro-RNAs can suppress multiple genes involved in these pathways. Total RNA was obtained from MDA-MB-231 cells after transfection with micro-RNA mimic, micro-RNA inhibitor, or non-targeting control MB231, performed in duplicate

2015-03-01 | E-GEOD-60766 | biostudies-arrayexpress

Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line. [sip53]

Project description:Mutant p53 can acquires oncogenic properties supporting tumor growth, metastases and chemoresistance, by reprogramming cancer cell transcriptome, proteome and metabolome. To investigate what is the gene expression network regulated by mutant p53, we silenced its expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231.

2023-10-06 | GSE214493 | GEO

MicroRNAome of highly metastatic and non-metastatic human breast cell lines

Project description:MicroRNAs are noncoding, endogenous small RNAs that regulate target genes by cleavage of the targeted mRNA or translational repression. We investigated the microRNAome using 2-color microarrays in a highly invasive human breast cancer cell line, MDA-MB-231 (sub line 4175) and a non-invasive breast epithelial cell line, MCF10A. We found 13 miRNAs that were up-regulated and 9 were down-regulated significantly in 4175 cells (p <0.05, fold change >2) compared with MCF10A cells. We compared the highly metastatic human breast cell lines MDA-MB-231 (4175 subline) with non-metastatic MCF10A cell lines. Two 4175 sublines and two MCF10A cell lines, independently grown and harvested. Dye swap was performed.

2010-05-15 | E-GEOD-17350 | biostudies-arrayexpress

IGDQ motogenic peptide gradient induces directional cell migration through integrin (αv)β3 activation in metastatic breast cancer

Project description:In the context of breast cancer metastasis study, we have shown in an in vitro model of cell migration that IGDQ-exposing (IsoLeu-Gly-Asp-Glutamine type I Fibronectin motif) monolayers (SAMs) on gold sustain the adhesion of MDA-MB-231 cells by triggering Focal Adhesion Kinase by activating integrins. Such tunable scaffolds are used to mimic the extracellular environment, inducing and controlling cell migration towards an anisotropic surface. The observed migratory behavior induced by the IGDQ-bearing peptide gradient along the surface suggests the presence of cell subpopulations: “stationary” or a “migratory” phenotype. We focused on the integrins α5(β1) and (αv)β3, already known to be implicated in cell migration. To this aim, a whole proteomic analysis was performed in beta 3 integrin (ITGB3) or alpha 5 integrin (ITGA5) knock-down MDA-MB-231, in order to highlight the pathways implied into the integrin-dependent cell migration. Our results showed that i) ITGB3 depletion influenced ITGA5 mRNA expression, ii) ITGB3 and ITGA5 were both necessary for IGDQ-mediated directional single cell migration, iii) integrin (αv)β3 was activated by IGDQ fibronectin type I motif and iv) co-regulation of retrograde transport of ITGB3 by ITGA5 is potentially necessary for directional IGDQ-mediated cell migration.

2022-11-08 | PXD024540 | Pride

Transcription profiling of human primary breast tumors and MDA-MB-231 subpopulations

Project description:Subpopulations of MDA-MB-231 that exhibit different metastatic tropisms when injected into immuno-deficient mice. Also, a cohort of primary breast cancers surgically resected at the Memorial Sloan-Kettering Cancer Center (MSKCC).

2008-06-12 | E-GEOD-2603 | biostudies-arrayexpress

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