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Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Nakaseomyces glabratus CBS 138 Nakaseomyces glabratus

PROVIDER: GSE23829 | GEO | 2010/12/31

SECONDARY ACCESSION(S): PRJNA130563

REPOSITORIES: GEO

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Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata.

Project description: Not available

| S-EPMC3052359 | biostudies-literature

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata

Project description:This SuperSeries is composed of the following subset Series: GSE23827: Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 1) GSE23828: Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 2) Refer to individual Series

2010-12-31 | E-GEOD-23829 | biostudies-arrayexpress

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 2)

2010-12-31 | GSE23828 | GEO

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 1)

2010-12-31 | GSE23827 | GEO

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 2)

Project description:In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles. Gene expression was measured in 2 C. glabrata clinical isolates (DSY717 and DSY2317). DSY2317 is azole-susceptible and contains a wild-type CgPDR1 allele. DSY717 is azole-resistant and contains a CgPDR1 allele with the gain-of-function mutation L1081F.

2010-12-31 | E-GEOD-23828 | biostudies-arrayexpress

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 1)

Project description:In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles. Gene expression was measured in 7 C. glabrata laboratory strains (SFY101, SFY103, SFY105, SFY109, SFY111, SFY115, SFY116) expressing different CgPDR1 hyperactive alleles, in 1 strain (SFY114) expressing a wild-type CgPDR1 allele and in one strain deleted for CgPDR1. The one-color system was used. 3 independent experiments were performed using 3 biological replicates for each strain.

2010-12-31 | E-GEOD-23827 | biostudies-arrayexpress

Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata

Project description:Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata

| PRJNA130563 | ENA

Molecular biological characterization of an azole-resistant Candida glabrata isolate.

Project description: Not available

| S-EPMC164098 | biostudies-other

Loss of mitochondrial functions associated with azole resistance in Candida glabrata results in enhanced virulence in mice.

Project description: Not available

| S-EPMC3088236 | biostudies-literature

Azole Resistance Mechanism in Candida glabrata

Project description:Microarray was used to analyze azole resistance of Candida glabrata oropharyngeal isolates from 7 hematopoietic stem cell transplant recipients receiving fluconazole prophylaxis. Transcriptional profiling of the sequential-paired clinical isolates by microarray revealed 19 genes upregulated in the majority of resistant isolates compared to their paired-susceptible isolates. All seven resistant isolates had greater than two fold upregulation of CgPDR1, a master transcriptional regulator of PDR network, and all 7 resistant isolates showed upregulation of known CgPDR1-target genes. The altered transcriptome can be explained in part by the observation that all 7 resistant isolates had acquired a single nonsynonymous mutation in their CgPDR1 ORF. Four mutations occurred in the regulatory domain (L280P, L344S, G348A, S391L) and one in the activation domain (G943S) while two mutations (N764I, R772I) occurred in an undefined region. Association of azole resistance and the CgPDR1 mutations was investigated in the same genetic background by introducing the CgPDR1 sequences from one sensitive and five resistant isolates into a laboratory azole-sensitive strain (cgpdr1) via integrative transformation. The cgpdr1 strain was restored to wild-type fluconazole susceptibility when transformed with CgPDR1 from the susceptible isolate but became resistant when transformed with CgPDR1 from the resistant isolates. However, despite the identical genetic background, upregulation of CgPDR1 and CgPDR1-target genes varied between the 5 transformants, independent of the domain locations in which the mutations occurred. In sum, gain-of-function mutations in CgPDR1 not only contributed to the clinical azole resistance but different mutations had varying degrees of impact on the CgPDR1-target genes.

2010-07-07 | GSE21355 | GEO

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