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18

Representative Illumina sequencing libraries upon T7-polymerase based amplification


ABSTRACT: This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Overall design: Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.

INSTRUMENT(S): Illumina Genome Analyzer II (Plasmodium falciparum 3D7)

ORGANISM(S): Plasmodium falciparum 3D7  

SUBMITTER: H.G. Stunnenberg   

PROVIDER: GSE23868 | GEO | 2010-10-28

SECONDARY ACCESSION(S): PRJNA133167

REPOSITORIES: GEO

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