Transcriptomics,Genomics

Dataset Information

16

Microarray analysis of gene expression profile in HCT116 colon cancer cells expressed the isoform A or isoform B of the Tazarotene-induced gene 1.


ABSTRACT: Tazarotene-induced gene 1 (TIG1), also named as retinoic acid receptor responder 1 (RARRES1), is a retinoid inducible type II tumor suppressor gene; the TIG1B isoform inhibits growth and invasion of cancer cells. Expression of TIG1B is frequently downregulated in various cancer tissues; however, the expression and activities of the TIG1A isoform has yet to be analyzed. This study investigated the effects of TIG1A and TIG1B isoforms on gene expression profiles of colon cancer cells. TIG1A, TIG1B and control stable clones derived from HCT116 colon cells were established using the GeneSwitch system. TIG1 isoform expression was induced upon 5 micro Molar of mifepristone (MFP) treatment for 24 hr. Biological triplicate samples were prepared and gene expression profiles were determined by microarray using human genome HGU133 plus 2 array (Affymatrix). Upon induction of TIG1A and TIG1B expression for 24 hr, a total of 129 and 55 genes were significantly altered, respectively. Of the genes analyzed, 23 and 6 genes were up- and downregulated, respectively in both TIG1A and TIG1B expressing cells. Overall design: TIG1A, TIG1B and control stable clones derived from HCT116 colon cells were established using the GeneSwitch system. Inducible stable clones were plated in 15-cm dishes overnight in maintaining medium free of hygromycin and zeocin. Cells were refreshed with medium containing 5 micro molar of mifepristone and then incubated overnight. Cells were washed with ice-cold PBS and then harvested. Total RNA was prepared. Biological triplicate samples were prepared for control, TIGA and TIG1B stable cells. Samples of cRNA were prepared, labeled with biotin, and then hybridized to Human Genome U133 Plus 2.0 Array.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Shun-Yuan Jiang  

PROVIDER: GSE23882 | GEO | 2011-08-15

SECONDARY ACCESSION(S): PRJNA130495

REPOSITORIES: GEO

altmetric image

Publications

G protein-coupled receptor kinase 5 mediates Tazarotene-induced gene 1-induced growth suppression of human colon cancer cells.

Wu Chang-Chieh CC   Tsai Fu-Ming FM   Shyu Rong-Yaun RY   Tsai Ya-Ming YM   Wang Chun-Hua CH   Jiang Shun-Yuan SY  

BMC cancer 20110517


BACKGROUND: Tazarotene-induced gene 1 (TIG1) is a retinoid-inducible type II tumour suppressor gene. The B isoform of TIG1 (TIG1B) inhibits growth and invasion of cancer cells. Expression of TIG1B is frequently downregulated in various cancer tissues; however, the expression and activities of the TIG1A isoform are yet to be reported. Therefore, this study investigated the effects of the TIG1A and TIG1B isoforms on cell growth and gene expression profiles using colon cancer cells. METHODS: TIG1A  ...[more]

Similar Datasets

| GSE92915 | GEO
| PRJNA106125 | ENA
2016-02-11 | BIOMD0000000591 | BioModels Database
2014-06-02 | E-GEOD-36588 | ArrayExpress
2015-01-02 | E-GEOD-58507 | ArrayExpress
2009-02-26 | GSE10650 | GEO
2011-06-10 | E-GEOD-10650 | ArrayExpress
| GSE69295 | GEO
2014-05-02 | E-TABM-461 | ArrayExpress
| GSE69294 | GEO