Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional response of USA300 MRSA to coculture with EGM139 Desemzia incerta

ABSTRACT: We wished to examine the effect that coculture with Desemzia incerta would have on the S. aureus transcriptional profile. We have previously shown D. incerta to have antimicrobial activity against S. aureus. We used a transwell coculture system to separate the two bacterial cultures. Transcriptional profiling revealed downregulated of genes in the arc operon, the pur operon, the mnh family proteins, and cap family proteins and upregulations in the arg operon and the ahp opeorn. Subsequent biological follow-up revealed decrease in S. aureus biofiom formation after D. incerta co-culture.

ORGANISM(S): Staphylococcus aureus

PROVIDER: GSE239513 | GEO | 2023/07/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Evolution of hypervirulence during bloodstream adaptation of CA-MRSA strain USA300

Project description:The epidemic community-acquired methicillin-resistant S. aureus (CA-MRSA) clone USA300 has recently become a leading cause of hospital-associated bloodstream infections (BSI). Leveraging this recent introduction into hospitals and the limited genetic variation across the USA300 strains, we combined microbial comparative genomics with phenotypic analyses to discover adaptive mutations. USA300 isolates from BSI were found to have independently evolved single nucleotide variants in the transcriptional regulator sarZ. sarZ inactivation lead to altered expression of virulence factors, resulting in increased lethality in a murine model of BSI. Thus, USA300 strains can optimize their fitness in hospitals through evolution of higher virulence.

2022-10-08 | GSE185544 | GEO

Stem cell coculture

Project description:This study aims to to compare the gene transcription profiles of endothelial cells and stem cells, when they are cultured alone or when they are cultured together. Thus there are two major questions - how do the cells differ, and how do the cells influence each other's gene expression. Thus there are 4 types of sample: endothelial cell monoculture, endothelial cell coculture, stem cells monoculture, stem cell coculture. There are also 4 biological replicates (independent experiments) leading to 16 array data files.

2013-10-10 | E-MTAB-1536 | biostudies-arrayexpress

Syntrophic coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCA

Project description:Propionate accumulation is an important bottleneck for anaerobic degradation of organic matter. We hypothesized that propionate conversion by a novel coculture of Syntrophobacter fumaroxidans and Geobacter sulfurreducens can be an alternative strategy for propionate oxidation coupled to Fe(III) reduction. In this study, we successfully cocultured S. fumaroxidans and G. sulfurreducens on propionate and Fe(III). Proteomic analyses of this coculture provided insights into the underlying mechanisms of propionate metabolism pathway and interspecies electron transfer mechanism. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems.

2022-02-17 | PXD027104 | Pride

Coculture of Desulfovibrio vulgaris Hildenborough and Methanococcus maripaludis

Project description:Using data from microarray experiments we investigated a coculture of D. vulgaris Hildenborough and Methanococcus maripaludis. Using data from microarray experiments we investigated a coculture of D. vulgaris Hildenborough and Methanococcus maripaludis.

2011-11-01 | E-GEOD-30831 | biostudies-arrayexpress

Association of methicillin-resistant Staphylococcus aureus (MRSA) USA300 genotype with mortality in MRSA bacteremia.

Project description: Not available

| S-EPMC2975870 | biostudies-literature

Gene expression analysis of Staphyloccoccus aureus USA300 vs USA300 sae knockout

Project description:The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300 vs USA300 sae knockout. Samples were hybridized on aminosilane coated slides with 70-mer oligos.

2011-04-08 | GSE26243 | GEO

Coculture of Desulfovibrio vulgaris Hildenborough and Methanococcus maripaludis

Project description:Using data from microarray experiments we investigated a coculture of D. vulgaris Hildenborough and Methanococcus maripaludis.

2011-11-01 | GSE30831 | GEO

circSamd4 represses myogenic transcriptional activity of PUR proteins

Project description:By interacting with proteins and nucleic acids, the vast family of mammalian circRNAs is proposed to influence many biological processes. Here, RNA sequencing analysis of circRNAs differentially expressed during myogenesis revealed that circSamd4 expression increased robustly in mouse C2C12 myoblasts differentiating into myotubes. Moreover, silencing circSamd4, which is conserved between human and mouse, delayed myogenesis and lowered the expression of myogenic markers in cultured myoblasts from both species. Affinity pulldown followed by quantitative mass spectrometry revealed that circSamd4 associated with PURA and PURB, two repressors of myogenesis that function by impairing transcription of the myosin heavy chain (MYH) protein family. Supporting the hypothesis that circSamd4 might complex with PUR proteins thereby preventing their interaction with DNA, silencing circSamd4 enhanced the association of PUR proteins with the Myh promoter, while overexpressing circSamd4 interfered with the binding of PUR proteins to the MYH promoter. These effects were abrogated when using a mutant circSamd4 lacking the PUR binding site. Our results indicate that the association of PUR proteins with circSamd4 directly contributes to myogenesis by derepressing MYH transcription.

2020-01-16 | GSE136004 | GEO

S. aureus gene expression of WT (USA300) vs fakAKO

Project description:S. aureus response to fakA deletion Gene expression profiles were generated by microarray analysis of S. aureus WT (USA300) or fakAKO

2014-08-01 | GSE57340 | GEO